海南医科大学学报2025，Vol.31Issue(12)：881-887,7.DOI:10.13210/j.cnki.jhmu.20241107.001
高糖环境下氧化三甲胺对骨髓源性巨噬细胞的影响研究
Effects of trimethylamine N-oxide on bone marrow-derived macrophages under high glucose conditions
摘要
Abstract
Objective:To investigate the effects of trimethylamine N-oxide(TMAO)on bone marrow-derived macrophages under high glucose conditions.Methods:Bone marrow-derived macrophages were subjected to different concentrations of TMAO using the CCK-8 assay to measure cell viability.Cells were divided into three groups:control,high glucose,and high glucose+TMAO.Immunofluorescence was employed to assess cellular activation states,while fluorescence quantitative PCR was used to detect IL-1β,IL-6 and TNF-α expression levels.Western blot was performed to evaluate AMPK,PINK1 and Parkin protein ex-pression levels.Results:Under high glucose conditions,TMAO showed no significant effect on the viability of bone marrow-de-rived macrophages(P>0.05).TMAO at 300 μmol/L significantly activated macrophage expression under high glucose conditions(P<0.05).Compared to the control group,levels of inflammatory factors IL-1β,IL-6,and TNF-α were elevated in both the high glucose and high glucose+TMAO groups(P<0.05).The protein expression of AMPK and PINK1 in the high glucose group was significantly lower than that in the control group(P<0.05),while the expression of Parkin showed no difference(P>0.05).In the high glucose+TMAO group,the protein expressions of AMPK,PINK1 and Parkin were all significantly decreased compared to the control group(P<0.05).Conclusion:TMAO does not affect the viability of bone marrow-derived macrophages under high glucose conditions but stimulates abnormal cellular activation and exacerbates inflammatory responses,its mechanisms potentially involve AMPK,PINK1,and Parkin.关键词
巨噬细胞/氧化三甲胺/高糖/炎症Key words
Macrophages/Trimethylamine N-oxide/High glucose/Inflammation分类
医药卫生引用本文复制引用
黄峥,戴霞,徐子岚,李苏蓝,吴妮晓..高糖环境下氧化三甲胺对骨髓源性巨噬细胞的影响研究[J].海南医科大学学报,2025,31(12):881-887,7.基金项目
This study was supported by the National Natural Science Foundation of China(82160440),Natural Science Foundation Project of Guangxi Province(2023GXNSFAA026323),Innovation Project of Guangxi Graduate Education(YCSW2024246) 国家自然科学基金资助项目(82160440) （82160440）
广西自然科学基金资助项目(2023GXNSFAA026323) （2023GXNSFAA026323）
广西研究生教育创新计划项目(YCSW2024246) （YCSW2024246）
