海南医科大学学报2025，Vol.31Issue(12)：928-937,10.DOI:10.13210/j.cnki.jhmu.20241209.001
结合生物信息学探究磷酸烯醇式丙酮酸羧激酶1对胆管癌细胞生物学行为的影响及机制
Exploring the effects and mechanisms of phosphoenolpyruvate carboxykinase 1 on the biological behavior of cholangiocarcinoma cells through bioinformatics
摘要
Abstract
Objective:To investigate the expression of phosphoenolpyruvate carboxykinase 1(PCK1)in cholangiocarcinoma cells,its effects on cell proliferation and migration,and the underlying mechanisms through bioinformatics analysis.Methods:Dif-ferentially expressed genes in cholangiocarcinoma were identified using mRNA sequencing data from the GEO database.A protein-protein interaction(PPI)network was constructed using STRING and Cytoscape,and key regulatory networks and genes were identified.The expression levels and pathological characteristics of key genes were validated using the TCGA and HPA databases.KEGG and GSEA enrichment analyses were performed to explore the functions of differentially expressed genes and PCK1-associ-ated pathways.RBE cholangiocarcinoma cells were transfected with lentivirus to overexpress PCK1.Transfection efficiency was confirmed by RT-qPCR and Western blot.Experimental groups included a negative control(RBE group),an empty vector con-trol(RBE-NC group),an overexpression group(RBE-OE group),and a PPARγ inhibitor group(RBE-OE+GW9662 group).Cell proliferation was assessed by colony formation and CCK8 assays,while migration was evaluated using wound healing and Transwell assays.Western blot was employed to analyze pathway protein expression.Results:Bioinformatics analysis revealed PCK1 as a key regulatory gene in cholangiocarcinoma,with low expression in tumor tissues and potential involvement in the PPAR signaling pathway.Cellular experiments showed that PCK1 mRNA and protein levels were significantly increased in RBE cells after transfection.Compared to the negative control,PCK1 overexpression inhibited RBE cell proliferation and migration,in-creased PPARγ and PTEN protein levels,and decreased p-mTOR/mTOR levels.These effects were reversed in the inhibitor group.Conclusion:PCK1 may act as a key regulatory gene in cholangiocarcinoma and potentially suppresses cell proliferation and migration through the activation of the PPARγ/PTEN/mTOR signaling pathway.关键词
生物信息学/磷酸烯醇式丙酮酸羧激酶1/胆管癌/生物学行为/机制Key words
Bioinformatics/Phosphoenolpyruvate carboxykinase 1/Cholangiocarcinoma/Biological behavior/Mechanism分类
医药卫生引用本文复制引用
严华悦,周汉旭,王胜威,杨立春,刘子祥,贾建光..结合生物信息学探究磷酸烯醇式丙酮酸羧激酶1对胆管癌细胞生物学行为的影响及机制[J].海南医科大学学报,2025,31(12):928-937,10.基金项目
This study was supported by the Key Natural Science Research Project of Anhui Provincial Colleges and Universities(2022AH051476),Key Natural Science Project of Bengbu Medical College(2021byzd197),and 2023 Graduate Research Innovation Program of Bengbu Medical College(Byycx23125) 安徽省高等学校自然科学研究重点项目(2022AH051476) （2022AH051476）
蚌埠医学院自然科学重点项目(2021byzd197) （2021byzd197）
蚌埠医学院2023年度研究生科研创新计划(Byycx23125) （Byycx23125）
