海洋渔业2025,Vol.47Issue(3):374-383,10.
饲料中添加南极磷虾粉对中华绒螯蟹雌蟹性腺特征、非特异性免疫及抗氧化功能的影响
Effects of feeding Antarctic krill meals on gonadal characteristics,non-specific immunity and antioxidant functions of female Eriocheir sinensis
摘要
Abstract
To investigate the effects of nutrient fortification on gonadal characteristics,non-specific immunity and antioxidant functions of female E.sinensis,three groups of artificial diets were added with0%,10%,20%Antarctic krill meals for 40 days,and fresh Sinonovacula constricta was fed as the control group.The gonadosomatic index and hepatopancreas somatic index of female E.sinensis in different diet groups were compared.The activities of non-specific immune enzymes such as acid phosphatase(ACP),alkaline phosphatase(AKP),phenol oxidase(PO),lysozyme(LZM),catalase(CAT),superoxide dismutase(SOD),and the contents of malondialdehyde(MDA)and hamocyanin(HCY)in the hemolymph of female E.sinensis in different periods were analyzed.The results showed that the hepatopancreas somatic index significantly decreased(P<0.05),the gonadosmatic index significantly increased in all experimental groups(P<0.05),and the 20%Antarctic krill meal diet group showed the highest result.The activities of ACP,AKP,PO,and LZM all increased gradually with the prolongation of nutrient fortification.Specifically,the activities of ACP,AKP,PO and LZM were the highest in the 20%Antarctic krill meal group on the 30th day,and there were significant differences with the control group(P<0.05).The activities of CAT,SOD,and MDA content in the hemolymph of all experimental groups generally decreased gradually with the prolongation of nutrient fortification,while the change in HCY content was not obvious.The activities of CAT,SOD,and the contents of MDA in the 20%Antarctic krill meal group were the lowest on the 30th day.Results showed that 10%-20%Antarctic krill meal can increase the gonadosomatic index,improve the non-specific immunity and antioxidant functions of female E.sinensis,and 20%addition is better.A period of 30 days of nutrient fortification is the appropriate time for female E.sinensis before release.关键词
中华绒螯蟹/南极磷虾粉/营养强化/非特异性免疫/抗氧化能力Key words
Eriocheir sinensis/Antarctic krill meal/nutrient fortification/non-specific immunity/antioxidant function分类
农业科技引用本文复制引用
谈如,黄晓荣,张涛,耿智,赵峰,冯广朋,庄平..饲料中添加南极磷虾粉对中华绒螯蟹雌蟹性腺特征、非特异性免疫及抗氧化功能的影响[J].海洋渔业,2025,47(3):374-383,10.基金项目
国家重点研发计划(2019YFD0901202) (2019YFD0901202)
上海市中华绒螯蟹现代农业产业技术体系项目(沪农科产字2017-4号) (沪农科产字2017-4号)
国家自然科学基金面上项目(31972795) (31972795)
中央级公益性科研院所基本科研业务费专项(2020TD13) (2020TD13)
国家海洋水产种质资源库资助项目 ()