吉林农业大学学报2025，Vol.47Issue(3)：407-414,8.DOI:10.13327/j.jjlau.2021.1612
大豆GmAP3基因的克隆及生物信息学分析
Cloning and Bioinformatics Analysis of Soybean GmAP3 Gene
摘要
Abstract
AP3 gene belongs to class B gene in MADS-box gene family,which can determine the for-mation of petals and stamens.It has been reported that AP3 gene is involved in regulating flowering time,flower meristem formation and ovule development.Based on the full-length cDNA sequence of soybean GmAP3 gene obtained by race technology in the early stage of the laboratory,we further ex-plored the function and expression regulation law of the gene.On the one hand,specific primers were designed to obtain its coding CDS sequence and construct the overexpression vector,the protein structure and function were analyzed by bioinformatics software and tools,and the protein interaction was predicted.On the other hand,the GmAP3 gene sequence was compared with the soybean genome sequence by snapgene software,the 1 646 bp promoter sequence upstream of the start codon of GmAP3 gene was successfully cloned,and its cis acting element was analyzed.The results showed that the full-length CDs sequence of GmAP3 gene was 623 bp,the molecular weight was 26 209.9 Da,the theoretical isoelectric point(pI)was 9.41,and there was no hydrophilic protein with signal pep-tide and transmembrane domain.The protein localization was predicted on the nucleus.The protein interaction prediction results showed that GmAP3 interacted with AP1,SEP,AG and other MADS family genes related to soybean flower development and seed formation.Promoter analysis showed that the gene was related to light signal response and jasmonic acid synthesis pathway.The results of binding site prediction showed that GmAP3 gene may work together with multiple flower develop-ment related genes to affect the growth of soybean flowers.关键词
大豆/GmAP3基因/MADS-box蛋白/启动子/生物信息学分析Key words
soybean/AP3 gene/MADS-box protein/promoter/bioinformatics analysis分类
农业科技引用本文复制引用
何浩博,赵秋竹,栾鑫超,张爱晶,李越,王丽雪,周俊名,姚丹..大豆GmAP3基因的克隆及生物信息学分析[J].吉林农业大学学报,2025,47(3):407-414,8.基金项目
吉林省自然科学基金项目(202002010227JC),吉林省重点研发计划项目(20210202006NC) （202002010227JC）
