吉林农业大学学报2025，Vol.47Issue(3)：415-421,7.DOI:10.13327/j.jjlau.2023.1976
人工老化大豆种子的幼苗生理特性及DNA甲基化变异
Physiological Characteristics and DNA Methylation Variation of Seedlings from Artificially Aged Soybean Seeds
摘要
Abstract
In order to explore the effects of aged seeds on physiological state and epigenetic inheri-tance of seedlings,two soybean varieties Jinong 43 and Jinong 36 were selected as experimental ma-terials.Seeds were artificially aged at(55±1)℃with relative humidity(RH)100%for 5,10 and 15 min,and untreated seeds were used as control.Physiological indexes of seedlings were deter-mined and DNA methylation variation at the 5'-CCGG site was analyzed.The results showed that with the extension of aging time,the activities of peroxidase(POD),superoxide dismutase(SOD)and catalase(CAT)of the aged soybean seeds showed a decreasing trend.When aging for 15 min,POD activity of the two cultivars was significantly lower than ck,by 32.9%and 14.5%,SOD activity was decreased by 16.4%and 22.2%,and CAT activity was decreased by ck 9.3%and 9.1%.The MDA content of aging soybean seeds increased with aging time,and the MDA content of"Jinong 43"and"Jinong 36"were 2.92 μmol/g and 3.23 μmol/g,respectively,after aging for 15 min.When the seeds were aged for 15 min,the 5'-CCGG DNA hemimethylation levels of single CHG and double stranded CG were increased with the aging degree,while the endogenous and exogenous cytosine methylation levels were decreased.The results indicated that artificial aging of soybean seeds could reduce the activity of physiologically active enzymes and change the overall methylation level of DNA 5'-CCGG site slightly,but the methylation level of different patterns changed greatly and showed different trends due to aging.关键词
大豆/种子老化/生理指标/DNA甲基化Key words
soybean/seed aging/physiological index/DNA methylation分类
农业科技引用本文复制引用
赵欣欣,孙明春,贾恩吉,刘光涛,赵仁贵..人工老化大豆种子的幼苗生理特性及DNA甲基化变异[J].吉林农业大学学报,2025,47(3):415-421,7.基金项目
吉林省科技发展计划项目(20200201121JC),吉林省教育厅"十三五"科学技术项目(JJKH20170309KJ) （20200201121JC）
