吉林农业大学学报2025，Vol.47Issue(3)：454-464,11.DOI:10.13327/j.jjlau.2024.0612
根皮苷对秀丽隐杆线虫的抗氧化和降脂作用
Lipid-lowering and Antioxidant Activities of Phlorizin on Caenorhab-ditis elegans
摘要
Abstract
We investigated the effects of phlorizin on the antioxidant and lipid-lowering activities of Caenorhabditis elegans by using phlorizin obtained from the leaves of Lithocarpus litseifolius(Hance)Chun in the laboratory.The effects of phlorizin on longevity,motility,stress resistance,antioxidant enzyme activity,lipofuscin accumulation,triglyceride and cholesterol content determination,and oil red O staining of C.elegans were determined and the mechanism of action was elaborated by using fluorescence quantitative PCR to reveal the anti-aging and lipid-lowering effects of phlorizin on C.el-egans.The results show the lifespan of nematodes treated with 50,100 and 200 μmol/L concentra-tions was improved under normal conditions,heat stress conditions and oxidative stress conditions,and was accompanied by an increase in motility.Phlorizin also increased the activities of SOD and CAT antioxidant enzymes and decreased the accumulation of MDA and lipofuscin.Triglyceride and cholesterol contents in nematodes were reduced after feeding phlorizin,while oil red O staining re-sulted in fewer red areas and smaller lipid droplet sizes.Phlorizin significantly up-regulated the ex-pression of daf-16,nhr-49,aak-2,acs-2 and atgl-1 genes,and significantly down-regulated the ex-pression of daf-2,fat-5,fat-6 and fat-7 genes.It indicates that phlorizin may reduce lipid accumu-lation through SREBP signalling pathway,nhr-49-mediated nuclear hormone signalling pathway,in-sulin signalling pathway,as well as AMPKsignalling pathway to enhance the motility and longevity of C.elegans.关键词
根皮苷/秀丽隐杆线虫/抗氧化/脂质代谢Key words
phlorizin/Caenorhabditis elegans/lipid metabolism/gene expression分类
农业科技引用本文复制引用
阮柳丁,谷奇,付玉杰..根皮苷对秀丽隐杆线虫的抗氧化和降脂作用[J].吉林农业大学学报,2025,47(3):454-464,11.基金项目
国家自然科学基金项目(31930076),江西省林业局林业科技创新专项[(2022)08号-2] （31930076）
