吉林农业大学学报2025，Vol.47Issue(3)：465-472,8.DOI:10.13327/j.jjlau.2023.1469
北细辛提取物对秀丽隐杆线虫的生物活性及机理研究
Bioactivity and Mechanism of Asarum heterotropoides Fr.Schmidt var.Mandshuricum(Maxim)Kitag.Extracts against Caenorhabditis elegans
摘要
Abstract
Asarum heterotropoides Fr.Schmidt,as a potential botanical pesticide,is increasingly gaining attention.In this study,Asarum heterotropoides Fr.Schmidt var.mandshuricum(Maxim)Kitag was used as material to explore the bioactivity effect of A.heterotropoldes extracts on Cae-norhabditis elegans.The results showed that the 24 h half lethal concentration(LC50)of the volatile oil of A.beisarensis against C.elegans was 907 mg/L,and its nematicidal activity was significantly better than that of petroleum ether layer extract(P<0.05).The average lifespan and maximum lifes-pan of nematodes in the volatile oil-exposed group were shortened by 27.71%and 25.00%,respec-tively,while the phenotypic parameters such as exercise capacity,body length and oviposition were significantly inhibited.Further biochemical analysis showed that volatile oil could synergistically in-hibit superoxide dismutase(SOD),catalase(CAT)and acetylcholinesterase(AChE),which are key defense enzyme systems of nematodes,resulting in increased lipofuscin accumulation and up-regulation of apoptosis.These results indicated that the toxic effect of the volatile oil was the result of the synergistic effects of developmental toxicity,reproductive toxicity,cytotoxicity and neurotoxicity.关键词
北细辛/秀丽隐杆线虫/生物活性/毒性机理Key words
Asarum heterotropoides/Caenorhabditis elegans/bioactivity/toxicity mechanism分类
农业科技引用本文复制引用
杨佳彤,焉学倩,赵洋,张志财,陈晓林,张恺新,王英平,张瑞..北细辛提取物对秀丽隐杆线虫的生物活性及机理研究[J].吉林农业大学学报,2025,47(3):465-472,8.基金项目
吉林省科技发展计划项目(20200404025YY) （20200404025YY）
