昆明医科大学学报2025，Vol.46Issue(6)：9-19,11.DOI:10.12259/j.issn.2095-610X.S20250602
URSA患者蜕膜PMN-MDSCs细胞基因表达特征及功能预测
Characteristics of Decidual PMN-MDSCs Gene Expression and Functional Prediction in URSA Patients
摘要
Abstract
Objective To investigate the gene expression characteristics and functional prediction of decidual polymorphonuclear myeloid-derived suppressor cells(PMN-MDSCs)in patients with unexplained recurrent abortion(URSA).Methods Decidual tissues were collected from 3 normal early pregnancy patients undergoing artificial abortion and 3 URSA patients at the First People's Hospital of Yunnan Province between July and December 2023.Magnetic-activated cell sorting(MACS)technology was used to separate decidual PMN-MDSCs,and gene expression differences were detected using gene chip technology.Differential genes were analyzed using DESeq2 or edgeR,controlling false positives with P-value and FDR.GO and KEGG analyses were performed to investigate the functional pathways of differential genes,and protein-protein interaction(PPI)network analysis and hub gene screening were conducted.Key genes were verified through immunofluorescence staining and quantitative analysis.Results Compared to the normal pregnancy group,a total of 163 genes in the decidual PMN-MDSCs of URSA patients showed significant changes(P<0.05),with 67 genes upregulated and 96 genes downregulated.GO and KEGG enrichment analyses revealed that these differential genes were involved in cellular components,biological processes,molecular functions,protein binding,complement system signaling pathways,leukocyte-mediated inflammatory response pathways,and proteoglycan and extracellular matrix receptor interactions.PPI network analysis and hub gene identification showed that among the top 10 hub genes,the upregulated genes were SPP1,CCL5,C3AR1,and TNF,while the downregulated genes included MXRA8,IGFBP5,SPARCL1,SAA1,DCN,and COL3A1.These hub genes were primarily associated with key biological processes such as immune regulation,inflammatory responses,and intercellular interactions.Immunofluorescence quantification results demonstrated that the expression level of SPP1 in decidual PMN-MDSCs of URSA patients was significantly higher than that in the normal pregnancy group,with a statistically significant difference(P<0.05).Conclusion PMN-MDSCs in URSA patients'decidual tissues exhibit functional abnormalities,characterized by weakened regulatory effects on extracellular matrix remodeling,reduced cell-cell interaction capabilities,decreased immunosuppressive capacity,and enhanced pro-inflammatory responses.This may be one of the important immunological mechanisms underlying pregnancy failure.关键词
不明原因复发性流产/髓系抑制细胞/母胎免疫耐受/生物信息学分析/GO富集分析/KEGG富集分析Key words
Unexplain recurrent spontaneous abortion/Myeloid derived suppressor cells/Maternal-fetal immune tolerance/Bioinformatics analysis/GO enrichment analysis/KEGG enrichment analysis分类
医药卫生引用本文复制引用
牛玉红,康晓敏..URSA患者蜕膜PMN-MDSCs细胞基因表达特征及功能预测[J].昆明医科大学学报,2025,46(6):9-19,11.基金项目
国家自然科学基金(82260311) （82260311）
