昆明医科大学学报2025，Vol.46Issue(6)：46-53,8.DOI:10.12259/j.issn.2095-610X.S20250606
肺炎链球菌感染小鼠CD4+T淋巴细胞亚群特征及功能分析
Characteristics and Functional Analysis of CD4+T Lymphocyte Subsets in Mice Infected with Streptococcus pneumoniae
摘要
Abstract
Objective To analyze the levels and functions of CD4+T cell subsets in mouse spleen and lung tissues after Streptococcus pneumoniae(S.P.)infection,and to explore the immune regulatory mechanisms S.P.infection.Methods Flow cytometry was used to detect the proportions of CD4+T cell subsets(Th1,Th2,Th17,and Treg cells)in mouse spleen tissues from control group(n=4),S.P.infection at 12 h(n=4),and 24 h(n=4).H&E staining was used to examine lung tissue pathological characteristics.Differential gene sets and functional changes in lung tissues were analyzed after S.P.infection for 2 and 5 days,and immune cell abundance was predicted.Results Significant inflammatory pathological features were observed in the lung tissues of mice after S.P.infection.The proportions of Th1 and Treg cells in the spleen tissues gradually increased after S.P.infection,with Th1 and Treg cell proportions significantly higher than the control group at 24 h post-infection(P<0.05).At 5 d post-infection,only Treg cell proportion was significantly higher than the control group(P<0.05).Functional analysis revealed abnormal activation of IL6,IL10,and IL4/IL13 signaling pathways 2 days after infection,and abnormal enrichment of IL-2 and IL-6/TGF-β pathways 5 days after infection.Conclusion Treg and Th1 cells are key immune regulatory cells in mice following S.P.infection.Modulating Treg cell function mediated by IL-10 and Th1 cell function mediated by IL-2 can improve immune responses after S.P.infection.关键词
肺炎链球菌/肺炎/CD4+T细胞/Treg细胞/Th1细胞/免疫调控Key words
Streptococcus pneumoniae/Pneumonia/CD4+T cell/Treg cell/Th1 cell/Immunoregulation分类
医药卫生引用本文复制引用
董越虹,赵昱,况轶群,贾杰..肺炎链球菌感染小鼠CD4+T淋巴细胞亚群特征及功能分析[J].昆明医科大学学报,2025,46(6):46-53,8.基金项目
云南省基础研究计划(202201AU070202) （202201AU070202）
