扬州大学学报（农业与生命科学版）2025，Vol.46Issue(3)：85-95,11.DOI:10.16872/j.cnki.1671-4652.2025.03.010
芍药花扣囊复膜酵母发酵液对H2O2诱导的细胞衰老模型抗衰老作用
The anti-aging effects of Paeonia lactiflora flowers fermented with Saccharomycopsis fibuligera on H2O2-induced cellular senescence model
摘要
Abstract
This study aimed to investigate the ameliorative effects of Saccharomycopsis fibuligera-fermented Paeonia lactiflora flower extract(SF)on cellular senescence and its underlying mechanisms,thereby providing a scientific foun-dation for developing natural anti-aging products.SF was prepared using the fermentation of S.fibuligera.The impact of the fermentation broth on NIH3T3 cell viability was assessed using the WST-1 cell viability assay kit.The intracellular reactive oxygen species(ROS)levels were detected with the DCFH-DA fluorescent probe.Mitochondrial membrane po-tential(MMP)was measured using the rhodamine 123(RH123)fluorescent probe.Nuclear damage was evaluated by propidium iodide(PI)fluorescence staining.Cell apoptosis was analyzed using AO/EB fluorescence staining and Annexin V-PI/FITC flow cytometry.Cell cycle distribution was determined by PI single staining combined with flow cytometry.Molecular docking was employed to study the interactions between the active components of P.lactiflora and anti-aging target proteins(SIRT1 and tyrosinase).The results showed that the SF exhibited no significant cytotoxicity to NIH3T3 cells at concentrations below 800 μg·mL-1.In the H2O2-induced cellular senescence model,the fermentation broth sig-nificantly improved cell morphology,reduced intracellular ROS levels,protected mitochondrial membrane potential,de-creased nuclear damage,lowered apoptosis rates,and reversed cell cycle arrest.Molecular docking results showed that benzoyloxypaeoniflorin from P.lactiflora had the strongest binding affinities with SIRT1 and tyrosinase,with binding energies of-52.16 and-46.55 kJ·mol-1,respectively,indicating its significant anti-aging potential.In conclusion,the SF possesses multiple roles in cell protection and anti-aging effects,which may be associated with the increased active components during fermentation,particularly gallic acid and benzoyloxypaeoniflorin.关键词
芍药花/扣囊复膜酵母发酵/抗衰老/细胞衰老模型/分子对接Key words
Paeonia lactiflora flower/Saccharomycopsis fibuligera fermentation/anti-aging/cellular senescence model/molecular docking分类
农业科技引用本文复制引用
尹佳欣,邬靖莲,金银哲,李冰,陶俊,金英善..芍药花扣囊复膜酵母发酵液对H2O2诱导的细胞衰老模型抗衰老作用[J].扬州大学学报（农业与生命科学版）,2025,46(3):85-95,11.基金项目
国家重点研发计划项目(2019YFD1001502) （2019YFD1001502）
