扬州大学学报（农业与生命科学版）2025，Vol.46Issue(3)：96-104,9.DOI:10.16872/j.cnki.1671-4652.2025.03.011
螺虫乙酯及其代谢物在家蚕体内多残留检测方法的建立及应用分析
Development and application analysis of multi-residue detection method for spirotetramat and its metabolites in silkworms
摘要
Abstract
Spirotetramat is effective in controlling piercing-sucking mouthparts pests,but in recent years,it has been found that insects and pests may develop resistance through metabolic adaptation.To investigate the metabolic character-istics of spirotetramat in insects,this study established a multi-residue analysis combining QuEChERS sample pretreat-ment method with liquid chromatography-tandem mass spectrometry(LC-MS/MS)under negative ion scanning and mul-tiple reaction monitoring modes.The method enabled simultaneous detection of spirotetramat and its four metabolites in silkworm tissues(midgut,silk glands,fat,blood,and feces).The results showed that using methanol as the extract sol-vent and 48 mg octadecyl bonded silica gel(C18)as the absorbent,the recoveries of the five target compounds ranged from 80.2％to 108.7％,with relative standard deviations between 0.5％and 9.9％.The limits of detection were 0.000 1-0.003 8 mg·kg-1,meeting the requirements of pesticide residue detection standards.In summary,this study successfully established a multi-residue analysis method for detecting spirotetramat and its four metabolites in silkworms.This method offers advantages such as simple and efficient pretreatment,minimal matrix interference,high sensitivity,and broad ap-plicability,thereby providing a scientific basis for investigating the metabolic processes of spirotetramat and its metabo-lites,as well as their distribution within silkworms.关键词
家蚕/螺虫乙酯/代谢产物/LC-MS/MS/多残留分析Key words
Bombyx mori/spirotetramat/metabolites/LC-MS/MS/multi-residue analysis分类
农业科技引用本文复制引用
赵明,徐笑然,王子建,肖雅琦,任莉,孟志远,陈小军..螺虫乙酯及其代谢物在家蚕体内多残留检测方法的建立及应用分析[J].扬州大学学报（农业与生命科学版）,2025,46(3):96-104,9.基金项目
江苏省重点研发计划(现代农业)项目(BE 2023327) （现代农业）
江苏省普通高校研究生实践创新计划项目(SJCX24_2283) （SJCX24_2283）
扬州市重点研发计划(现代农业)项目(YZ 2023038) （现代农业）
