现代畜牧兽医Issue(6)：7-11,5.DOI:10.20154/j.cnki.issn1672-9692.2025.06.002
中华蜜蜂Peter Pan蛋白的原核表达载体构建
Construction of prokaryotic expression vector of Peter Pan protein of Apis cerana
摘要
Abstract
This study aimed to investigate the role of Peter Pan(PPAN)in Chinese honeybee(Apis cerana)larvae.A prokaryotic expression plasmid was constructed to induce PPAN protein expression.The PPAN gene was amplified using PCR,and the amplified product was inserted into the prokaryotic expression vector pGEX-4T-1.The recombinant plasmid pGEX-4T-1-PPAN was successfully constructed and transformed into Escherichia coli BL21(DE3)competent cells.Optimal induction conditions for the target protein were analyzed via SDS-PAGE,and Western blot was performed for verification.Results demonstrated that PPAN protein was successfully expressed in inclusion body form in Escherichia coli.The optimal induction conditions were determined as follows:Induction temperature 28℃,induction time 8 h,and isopropyl β-D-1-thiogalactopyranoside(IPTG)concentration of 0.5 mmol/L.The amplified PPAN gene fragment measured 1 146 bp,encoding a protein with a molecular weight of 70.6 kDa.This study successfully established a prokaryotic expression system for PPAN protein in Escherichia coli,laying a foundation for further investigation into the impact of PPAN protein on Chinese Sacbrood Virus(CSBV)replication.关键词
中华蜜蜂幼虫/原核表达/彼得潘(PPAN)Key words
Apis cerana larva/Prokaryotic expression/Peter Pan(PPAN)分类
农业科技引用本文复制引用
仇会桐,刘宇明,白桦,程玉,李明,费东亮,刘艳霞,马鸣潇..中华蜜蜂Peter Pan蛋白的原核表达载体构建[J].现代畜牧兽医,2025,(6):7-11,5.基金项目
国家自然科学基金(31772760) （31772760）
