现代畜牧兽医Issue(6)：28-33,6.DOI:10.20154/j.cnki.issn1672-9692.2025.06.006
调控PRRSV复制的功能性长链非编码RNA的筛选与鉴定
Screening and identification of functional long non-coding RNAs regulating porcine reproductive and respiratory syndrome virus replication
摘要
Abstract
To screen host long non-coding RNAs(lncRNAs)capable of regulating porcine reproductive and respiratory syndrome virus(PRRSV)replication,the study collected samples from PRRSV-infected Marc-145 cells at 24 h and 48 h post-infection.Whole-transcriptome sequencing analysis was performed using the BGI second-generation sequencing platform to identify significantly altered lncRNA candidate molecules following viral infection.After initial validation,LTCONS_00092659(designated as NRPL)was selected for functional investigation.Knockdown and overexpression approaches were employed to examine NRPL's impact on PRRSV replication.Results demonstrated that NRPL knockdown significantly inhibited PRRSV replication,whereas NRPL overexpression markedly promoted viral replication.This study reveals that lncRNA-NRPL critically facilitates PRRSV infection,suggesting its potential as a novel host target for developing anti-PRRSV therapies.These findings provide a promising therapeutic target for controlling Porcine Reproductive and Respiratory Syndrome.关键词
猪繁殖与呼吸综合征病毒/长链非编码RNA/转录组学/Ⅰ型干扰素Key words
Porcine reproductive and respiratory syndrome virus(PRRSV)/Long non-coding RNAs(lncRNAs)/Transcriptomics/IFN-Ⅰ分类
农业科技引用本文复制引用
刘宇明,许大伟,马鸣潇,李明..调控PRRSV复制的功能性长链非编码RNA的筛选与鉴定[J].现代畜牧兽医,2025,(6):28-33,6.基金项目
辽宁省博士科研启动基金计划项目(2023-BS-198) （2023-BS-198）
锦州医科大学博泽青年科技人才托举项目(JYQT202203) （JYQT202203）
