实验技术与管理2025，Vol.42Issue(6)：218-225,8.DOI:10.16791/j.cnki.sjg.2025.06.028
医用硅胶多功能抗菌涂层的实验设计和教学应用
Experimental design and teaching application of multi-functional antibacterial coatings for medical silicone
摘要
Abstract
[Objective]This study aims to design a comprehensive experimental teaching platform that integrates cutting-edge research on antibacterial materials into undergraduate education.By developing a multifunctional antibacterial coating for medical silicone,the experiment seeks to enhance students'practical skills,critical thinking,and interdisciplinary knowledge integration while addressing clinical challenges such as catheter-associated infections.The pedagogical goal is to bridge scientific research and education,fostering innovation in biomedical engineering.[Methods]A polydopamine(PDA)-modified silicone substrate was synthesized,incorporating Au@MnO2 nanocomposites to achieve synergistic photothermal and catalytic antibacterial effects.The coating preparation involved PDA self-polymerization,Mn2+chelation,and in situ growth of Au@MnO2 nanostructures.Characterization techniques,including SEM,XRD,Raman spectroscopy,and XPS,were employed to analyze the coating's morphology and composition.Antibacterial performance was evaluated against E.coli under near-infrared(NIR)irradiation,with hydroxyl radical(·OH)generation quantified via fluorescence spectroscopy and electron paramagnetic resonance(EPR).The experiment was embedded into a teaching framework,guiding students through hypothesis formulation,data analysis,and mechanism exploration.[Results]The Au@MnO2/PDA/silicone coating demonstrated exceptional antibacterial efficacy,achieving a 100%inhibition rate against E.coli under NIR irradiation(0.6 W/cm2,10 min).Structural analysis confirmed uniform dispersion of Au@MnO2 nanoparticles on the PDA matrix,with enhanced light absorption(400-1 200 nm)and localized temperature rise up to 50℃.The synergistic mechanism combined photothermal effects and nanozyme-catalyzed ROS generation,validated by·OH detection via TMB oxidation and EPR.Students successfully replicated experimental protocols,analyzed data trends,and proposed optimization strategies,demonstrating improved problem-solving and interdisciplinary competencies.[Conclusions]This study establishes a robust science-education integration model by transforming advanced antibacterial material research into an immersive teaching experiment.The Au@MnO2/PDA coating offers a promising solution for reducing medical device-related infections,while the pedagogical design cultivates students'innovation capabilities and scientific literacy.The experiment's success highlights the value of integrating research frontiers into curricula to train interdisciplinary talent for healthcare challenges.关键词
科教融合/医用硅胶/抗菌涂层/纳米酶/实验教学Key words
integration of science and education/medical silicone/antibacterial coatings/nanozymes/experimental teaching分类
社会科学引用本文复制引用
邱云峰,李信哲,丁凤鸣,马卓,王义铭,于淼,刘绍琴,王友善..医用硅胶多功能抗菌涂层的实验设计和教学应用[J].实验技术与管理,2025,42(6):218-225,8.基金项目
国家级大学生创新创业训练计划项目"聚多巴胺|MnO2-Au复合医用导管涂层的制备和抗菌机理研究"(202410213169) （202410213169）
