摘要
Abstract
Objective To explore the mechanism of Xiaoji Yinzi in the treatment of bladder cancer based on network pharmacology,molecular docking,and in vitro experiments.Methods ①Potential targets of Xiaoji Yinzi related to bladder cancer were screened using the TCMSP and GeneCards databases.A network of"herbal medicine-active components-targets-pathways-disease"was con-structed using Cytoscape 3.10.2 software.Molecular docking technology was applied to validate the affinity between key active compo-nents and core targets obtained from the network.②TCCSUP cells were respectively treated with different concentrations of quercetin(50-150 μmol/L)and kaempferol(100-200 μmol/L),and the inhibitory rate was detected by MTT assay.TCCSUP cells were divided into blank control group,quercetin(75,100,125 μmol/L)groups,and kaempferol(150,175,200 μmol/L)groups,and then the ex-pressions of ESR1/MAPK pathway proteins were detected.Results ①A total of 109 active components of Xiaoji Yinzi,including robinin and quercetin,and 84 potential targets for bladder cancer were identified through network pharmacology.Molecular docking re-sults showed that quercetin,kaempferol,licochalcone A,acacetin,and formononetin had good affinity with PTGS2,ESR1,HSP90AA1,AR,and PPARG.②Quercetin and kaempferol significantly reduced the viability of bladder cancer cells in a dose-depen-dent manner(P＜0.05).Quercetin and kaempferol inhibited the expressions of ESR1 and MAPK proteins in dose-dependent patterns(P＜0.05).Conclusion The herbal formula Xiaoji Yinzi may inhibit bladder cancer cell proliferation through its core bioactive compo-nents,such as quercetin and kaempferol,and can exert anti-bladder cancer effects by targeting the ESR1 and MAPK signaling axes.关键词
小蓟饮子/膀胱癌/网络药理学/中药/分子对接/细胞实验Key words
Xiaoji Yinzi/bladder cancer/network pharmacology/Chinese mdicine/molecular docking/cell experiments分类
医药卫生
