山西医科大学学报2025，Vol.56Issue(5)：495-501,7.DOI:10.13753/j.issn.1007-6611.2025.05.005
细胞角蛋白19对人宫颈癌细胞增殖及β-catenin核转位的影响
Effect of cytokeratin 19 on proliferation and nuclear translocation of β-catenin in human cervical cancer cells
摘要
Abstract
Objective To investigate the molecular mechanism of cytokeratin 19(KRT19)regulating cervical cancer cell proliferation through β-catenin signaling.Methods The expression level of KRT19 in cervical cancer and other malignancies was analyzed using the GEPIA online database.HeLa-KRT19(stable KRT19 overexpression)and SiHa-shKRT19(stable KRT19 knockdown)cell lines were established via puromycin selection.Western blotting and immunocytochemistry were employed to detect the expressions of KRT19,β-catenin,and Cyclin D1 in HeLa-KRT19 and SiHa-shKRT19 cells.Cell counting,MTT assays and flow cytometry were used to analyze cell proliferation,cell viability and cell cycle in HeLa-KRT19 and SiHa-shKRT19 cells.Results The results of GEPIA online database analysis revealed significantly elevated KRT19 expression in cervical cancer tissues compared to normal tissues(P＜0.05).KRT19 was lowly expressed in HeLa cells but highly expressed in SiHa cells.The stable HeLa-KRT19 and SiHa-shKRT19 cell lines were successfully constructed.Compared with HeLa-GFP cells,β-catenin nuclear translocation was enhanced in HeLa-KRT19 cells,Cyclin D1 expression was upregulated(P＜0.05),G0/G1 phase cell percentage was reduced(P＜0.05),and the prolifera-tion was increased(P＜0.05).Compared with SiHa-GFP cells,β-catenin nuclear translocation was suppressed in SiHa-shKRT19 cells,Cyclin D1 was down regulated(P＜0.05),G0/G1 phase cell percentage was increased(P＜0.05),and the cell proliferation was decreased(P＜0.05).Conclusion KRT19 can upregulate Cyclin D1 protein expression by facilitating β-catenin nuclear translocation,thereby promoting the proliferation of cervical cancer cells in vitro.关键词
细胞角蛋白19/β-catenin/核转位/细胞增殖/宫颈癌/细胞周期Key words
KRT19/β-catenin/nuclear translocation/cell proliferation/cervical cancer/cell cycle分类
医药卫生引用本文复制引用
张园,刘宪,张巧瑞,王蕾,崔南..细胞角蛋白19对人宫颈癌细胞增殖及β-catenin核转位的影响[J].山西医科大学学报,2025,56(5):495-501,7.基金项目
国家自然科学基金资助项目(81903042) （81903042）
陕西省自然科学基础研究计划面上项目(K2023-JC-YB-697) （K2023-JC-YB-697）
宁夏自然科学基金项目(2022AAC03536) （2022AAC03536）
宁夏医科大学科学研究基金项目(XZ2020022) （XZ2020022）
