中国临床药理学杂志2025，Vol.41Issue(9)：1236-1241,6.DOI:10.13699/j.cnki.1001-6821.2025.09.007
西达本胺抑制食管鳞癌细胞侵袭和上皮间质转化的研究
Research on chidamide suppressing invasion and epithelial-mesenchymal transition in esophageal squamous cell carcinoma
摘要
Abstract
Objective To explore the mechanism of inhibiting invasion and epithelial mesenchymal transformation of esophageal squamous cell carcinoma cells by chidamide(CHI)through long noncoding RNA(LncRNA)VPS9D1-AS1.Methods Human normal esophageal epithelial cells(HEEC)group(HEEC were cultured normally without any treatment),human esophageal squamous cell carcinoma KYSE-450 cells were divided into KYSE-450 group(normal culture),CHI group(treated with 20 μmol·L-1 CHI for 48 h),CHI+oe-NC group(transfected with oe-NC,treated with 20 μmol·L-1 CHI for 48 h),CHI+oe-LncRNA VPS9D1-AS1 group(transfected with oe-LncRNA VPS9D1-AS 1,treated with 20 μmol·L-1 CHI for 48 h).The positive expression of LncRNA VPS9D1-AS1in the cells was detected by fluorescence in situ hybridization,the invasion ability of the cells was detected by Transwell,and the epithelial mesenchymal transformation and mitogen-activated protein kinase(MEK)/extracellular signal-related protein kinase(ERK)signaling pathway related protein expression levels were detected by protein Western blot.Results The positive expression levels of LncRNA VPS9D1-AS1 in HEEC group,KYSE-450 group and CHI group were 1.00±0.06,4.58±0.84 and 1.93±0.37,respectively.Compared with HEEC group and KYSE-450 group,compared with CHI group and KYSE-450 group,the positive expression levels of LncRNA VPS9D1-AS1 were significantly different(P＜0.05).The invasion numbers of cells in KYSE-450 group,CHI group,CHI+oe-NC group,CHI+oe-LncRNA VPS9D1-AS1 group were 89.57±15.61,22.49±5.18,27.04±5.95 and 57.82±10.73,respectively;the relative expression levels of N-cadherin were 1.00±0.07,0.52±0.12,0.59±0.09 and 0.91±0.16,respectively;the relative expression levels of E-cadherin were 1.00±0.11,1.81±0.35,1.79±0.30 and 1.24±0.22,respectively;the relative expression levels of MEK1 were 1.00±0.08,0.64±0.11,0.69±0.13 and 0.93±0.17,respectively;p-ERK1/ERK1 levels were 1.00±0.05,0.61±0.07,0.55±0.10 and 0.95±0.15,respectively;p-ERK2/ERK2 levels were 1.00±0.12,0.43±0.09,0.42±0.08 and 0.89±0.14,respectively.Compared between KYSE-450 group and CHI group,between CHI+oe-LncRNA VPS9D1-AS1 group and CHI+oe-NC group,there were statistical significance in the levels of the above indexes(P＜0.05).Conclusion CHI can inhibit esophageal squamous cell invasion and epithelial mesenchymal transformation,which may be related to the inhibition of MEK/ERK signaling pathway activation by LncRNA VPS9D1-AS1 down-regulated by CHI.关键词
西达本胺/食管鳞癌/上皮间质转化/N-钙黏蛋白/有丝分裂原活化蛋白激酶Key words
chidamide/esophageal squamous cell carcinoma/epithelial mesenchymal transformation/N-cadherin/mitogen-activated protein kinase分类
医药卫生引用本文复制引用
冯博,陈伟霞,许彦超,曹家瑞,马纯政..西达本胺抑制食管鳞癌细胞侵袭和上皮间质转化的研究[J].中国临床药理学杂志,2025,41(9):1236-1241,6.基金项目
河南省重点研发与推广专项(科技攻关)基金资助项目(232102310126) （科技攻关）
