中国临床药理学杂志2025，Vol.41Issue(9)：1242-1247,6.DOI:10.13699/j.cnki.1001-6821.2025.09.008
人参皂苷Rh2影响结肠癌发展的研究
Research of ginsenoside Rh2 affecting the development of colon cancer
摘要
Abstract
Objective To explore the effects of ginsenoside Rh2 on the development of colon cancer through microRNA-128-3p(miR-128-3p)/growth differentiation factor 15(GDF15)mediated wingless-type MMTV integration site family member(Wnt)/β-catenin signaling pathway.Methods SW620 colorectal cancer cells were randomly divided into the following groups:Control group(standard culture),experimental group(treated with ginsenoside Rh2 at 20 μmol·L-1),NC inhibitor group(transfected with NC inhibitor),miR-128-3p inhibitor group(transfected with miR-128-3p inhibitor),ginsenoside Rh2+miR-128-3p inhibitor group(treated with ginsenoside Rh2 at 20 μmol·L-1 and transfected with miR-128-3p inhibitor),and ginsenoside Rh2+miR-128-3p inhibitor+si-GDF15 group(treated with ginsenoside Rh2 at 20 μmol·L-1,transfected with miR-128-3p inhibitor and si-GDF15).Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the relative expression levels of miR-128-3p and GDF15 mRNA.Cell proliferation was assessed using 5-ethynyl-2′-deoxyuridine(EdU)incorporation assay.Western blotting was employed to detect the protein relative levels of myelocytomatosis oncogene(Myc)and β-catenin.Results The relative expression levels of miR-128-3p in the control group and experimental group were 1.00±0.16 and 1.56±0.25,respectively;the relative expression relative levels of GDF15 mRNA were 1.00±0.12 and 0.43±0.05,respectively;the proliferation rates were(88.76±8.56)％and(35.89±4.05)％,respectively;the v-myc avian myelocytomatosis viral oncogene homolog(Myc)protein levels were 1.00±0.21 and 0.45±0.06,respectively;and the β-catenin protein relative levels were 1.00±0.23 and 0.52±0.07,respectively.Compared with the control group,the above indicators in the experimental group showed statistically significant differences(all P＜0.05).In the ginsenoside Rh2+miR-128-3p inhibitor group and ginsenoside Rh2+miR-128-3p inhibitor+si-GDF15 group,the cell proliferation rates were(68.56±7.12)％and(50.56±5.18)％,respectively;the Myc protein relative levels were 0.86±0.12 and 0.61±0.08,respectively;and the β-catenin protein relative levels were 0.71±0.09 and 0.58±0.08,respectively.The above indicators in the ginsenoside Rh2+miR-128-3p inhibitor group compared with the experimental group,and those in the ginsenoside Rh2+miR-128-3p inhibitor+si-GDF15 group compared with the ginsenoside Rh2+miR-128-3p inhibitor group,all showed statistically significant differences(all P＜0.05).Conclusion Ginsenoside Rh2 can inhibit the proliferation and migration of colon cancer SW620 cells,and promote the apoptosis,which may be related to the regulation of ginsenoside Rh2 on the miR-128-3p/GDF15 to inhibit Wnt/β-catenin signaling pathway.关键词
人参皂苷Rh2/微小核糖核酸-128-3p/结肠癌/增殖/迁移/凋亡/β-连环素/生长分化因子15Key words
ginsenoside Rh2/microRNA-128-3p/colon cancer/proliferation/migration/apoptosis/β-catenin/growth differentiation factor 15分类
医药卫生引用本文复制引用
易建中,刘云庚,刘传渊,肖荷芳,张磊,刘红权,方传发..人参皂苷Rh2影响结肠癌发展的研究[J].中国临床药理学杂志,2025,41(9):1242-1247,6.基金项目
江西省卫生健康委科技计划基金资助项目(20204628) （20204628）
