中国农业科技导报2025，Vol.27Issue(7)：30-43,14.DOI:10.13304/j.nykjdb.2024.0621
小麦PHY基因家族鉴定及热胁迫下表达分析
Identification of PHY Gene Family in Wheat(Triticum aestivum L.)and Their Expression Analysis Under Heat Stress
摘要
Abstract
Phytochrome(PHY)in plants not only receives light signals as a photoreceptor,but also responds to environmental temperature changes as a temperature sensor.To identify PHY genes in wheat,the bioinformatics methods was used,and their physical and chemical properties,phylogenetic evolution,gene structure,protein conserved domain,promoter cis-acting element and gene expression characteristics were comprehensively analyzed.The results showed that 9 TaPHY genes were identified,which randomly distributed on 6 chromosomes of wheat.And the subcellular localization prediction showed that these proteins were located in the nucleus.Phylogenetic analysis showed that these genes were classified into 3 subclasses,the genes of same subclass had high similarity of gene structure and protein conserved domain,which suggested similar function.Cis-element analysis revealed that the promoter of TaPHY genes had a variety of light and hormone response elements.In addition,tissue expression analysis identified significant differences in the expression abundance of TaPHY genes.Combined with the screening results of heat tolerant and fluorescence quantitative analysis,the expression levels of TaPHYA1 and TaPHYC2 were first down-regulated and then up-regulated under heat stress condition,while other genes showed different degrees of down-regulation,it showed that TaPHY genes induced by heat stress and participated in the regulation of heat resistance of wheat.Correlation analysis showed that the expression of TaPHYA3 was significantly negatively correlated with H2O2 content in the heat-resistant material 1-2-3,TaPHYC1 was positively correlated with superoxide dismutase(SOD)activity,TaPHYC3 expression was significantly correlated with SOD activity.Above results provided an experimental basis for further understanding the mechanism of TaPHY involved in heat stress response,and also provided important genetic resources for the screening of heat tolerant wheat lines.关键词
小麦/PHY基因家族/基因鉴定/热胁迫/相关分析Key words
wheat/PHY gene family/gene identification/heat stress/correlation analysis分类
农业科技引用本文复制引用
胡懿,公杰,赵玮,程蓉,柳忠玉,高世庆,杨亚珍..小麦PHY基因家族鉴定及热胁迫下表达分析[J].中国农业科技导报,2025,27(7):30-43,14.基金项目
北京市科技新星交叉项目(20220484204) （20220484204）
北京市农林科学院创新能力建设专项(KJCX20230203) （KJCX20230203）
农业生物育种国家科技重大专项(2023ZD040230204) （2023ZD040230204）
作物表型组学协同创新中心项目(KJCX20240406). （KJCX20240406）
