中国农业科技导报2025，Vol.27Issue(7)：44-53,10.DOI:10.13304/j.nykjdb.2024.0885
外源基因插入位点对抗虫棉Bt蛋白含量的影响
Effect of Exogenous Gene Insertion Site on Bt Protein Content in Insect-resistant Cotton
摘要
Abstract
The insertion sites of exogenous genes can affect the gene expression and character expression of recipient materials.In order to determine the effect of exogenous gene insertion sites on Bt protein content of insect-resistant cotton,29 insect-resistant cotton lines were selected for Bt protein content determination and whole genome resequencing analysis.The results showed that there were significant differences in Bt protein content among different lines,and the K3 line was the highest.Whole-genome resequencing analysis and PCR identification revealed that the Bt gene was a single copy in 29 lines,but the insertion sites were different.The Bt gene of K3 line was located in the 2 791 303～2 791 335 bp of A02 chromosome,while the Bt gene of other lines were located in D12 chromosome.To further verify the effect of insertion site on Bt protein content,the K3 line was hybridization with control Xinmian 33B,and F2 population was constructed.The results showed that the Bt protein content of F2 individual plants with the insertion site of Bt gene in A02 chromosome was significantly higher than that D12 type individual plants.In summary,the Bt protein content was affected by the insertion site of Bt gene,and its location on chromosome A02 was more conducive to the efficient expression of Bt gene.Above results could provide germplasm resources and theoretical support for breeding new varieties of insect-resistant cotton.关键词
抗虫棉/外源基因/插入位点/Bt蛋白Key words
insect-resistant cotton/exogenous gene/insertion site/Bt protein分类
农业科技引用本文复制引用
赵贵元,王永强,刘建光,耿昭,张寒霜,吴立强,王省芬,张桂寅..外源基因插入位点对抗虫棉Bt蛋白含量的影响[J].中国农业科技导报,2025,27(7):44-53,10.基金项目
农业生物育种重大项目(2023ZD04039-05) （2023ZD04039-05）
国家棉花产业技术体系建设项目(CARS-15-03) （CARS-15-03）
河北省棉花产业协同创新中心建设项目 （）
河北省省级科技计划项目(21326314D) （21326314D）
河北省现代农业产业技术体系棉花产业创新团队项目(HBCT2024100203) （HBCT2024100203）
河北省农林科学院创新工程项目(2022KJCXZX-MHS-5). （2022KJCXZX-MHS-5）
