中国农业科学2025，Vol.58Issue(12)：2275-2290,16.DOI:10.3864/j.issn.0578-1752.2025.12.001
耐盐南粳品种苗期盐胁迫应答的生理特性及其耐盐相关基因分析
Physiological Characteristics in Response to Salt Stress and Allelic Variation and Expression of Salt-Responsive Genes in Seedling Stage of Nangeng Rice Varieties with Salt-Tolerance Ability
摘要
Abstract
[Objective]Salt stress is one of the main environmental stresses that restrict rice production.Studying the physiological characteristics under salt stress and analysis the allelic variation and expression of salt-tolerance genes provide key gene resources and genetic materials for breeding salt-tolerance rice varieties.[Method]This study first evaluated the salt-tolerance ability of the Nangeng series high-quality rice varieties/lines during the seedling stage,using survival rate as an indicator for screening salt-tolerance varieties,which physiological changes under salt stress were analyzed,including chlorophyll,Na+,K+,MDA,H2O2 and soluble sugar.The variation types and expression levels of salt-tolerance genes in rice varieties with resistance to high salt concentration were also analyzed to explaining their molecular mechanisms in response to salt stress.[Result]Under the condition of treating with 140 mmol·L-1 NaCl for 6 days,the survival rates of NG9108,NG5718,and NGY1 were greater than 60%,with the highest survival rate among the tested varieties.Compared with Nipponbare,the seedlings of NG9108,NG5718,and NGY1 under salt stress had higher chlorophyll content and lower MDA content,indicating that salt stress caused less cell damage to the three varieties.The Na+/K+values in the roots of NG9108,NG5718,and NGY1 were significantly higher than those in Nipponbare,while the Na+/K+values in the aerial parts were significantly lower than those in Nipponbare,implying that the three varieties absorb or store more Na+in roots,but transport less Na+upwards,which is beneficial for maintaining cell ion balance and causing less ion toxicity and osmotic stress in aerial parts of the seedlings.The three salt-tolerance varieties have 94 SNPs or InDel sites,distributing in exons,introns,5′UTR,and 3′UTR of the 23 salt-tolerance genes.24 variation sites of 11 genes occur in the exons,including 7 genes with frameshift mutations or missense mutations which distributed in Os02g0813500(OsGR2),Os05g0343400(OsWRKY53),Os06g0685700(OsRST1),Os07g0685700(OsEIL2),Os10g0431000(OsPQT3),Os11g044600(OsRSS3),Os12g0150200(P450).Salt stress significantly induces expression of OsSKC1,OsBAG4,OsGPX1,OsCCX2,OsGR3,OsDREB2a,OsRAB21,OsP5CS,OsbZIP23,OsAPX37 and OsLEA3,which help to enhance salt tolerance and reduce the adverse effects of salt damage on rice growth.[Conclusion]NG9108,NG5718 and NGY1 showed strong salt tolerance phenotype during the seedling growth stage,which is closely related to the balance of sodium and potassium ions under salt stress,allelic variations of multiple salt tolerance genes,and gene expression levels.NG9108,NG5718 and NGY1 have pyramided multiple salt tolerant and high-quality genes,which can be used as backbone parents for genetic improvement and breeding.关键词
南粳9108/南粳5718/南粳盐1号/盐胁迫/耐盐基因/离子平衡/基因组测序Key words
NG9108/NG5718/NGY1/salt stress/salt-tolerance gene/ionic balance/genome sequencing引用本文复制引用
邓立成,李程,赫磊,安红强,王才林,张亚东,赵长江,路凯..耐盐南粳品种苗期盐胁迫应答的生理特性及其耐盐相关基因分析[J].中国农业科学,2025,58(12):2275-2290,16.基金项目
国家重点研发计划(2023YFD1200900)、江苏省重点研发计划(BE2023355) （2023YFD1200900）
