中国中医眼科杂志2025，Vol.35Issue(7)：601-606,6.DOI:10.13444/j.cnki.zgzyykzz.2025.07.001
青蒿琥酯缓释制剂抑制急性前葡萄膜炎模型兔眼前节炎症的实验研究
Experimental Study on the Inhibitory Effect of Artemether Sustained-release Prepa-ration on Anterior Segment Inflammation in a Rabbit Model of Acute Anterior Uveitis
摘要
Abstract
OBJECTIVE To investigate the effect of an artemether sustained-release preparation on anterior segment inflammation in a rabbit model of acute anterior uveitis.METHODS An endotoxin-induced uveitis(EIU)model was established in New Zealand rabbits by intravitreal injection of lipopolysaccharide(LPS).Rabbits were randomly divided into the model group(MG),triamcinolone acetonide group(TA),and artemether group(ART),with a Sham-operated group(Sham)receiving 0.9%sodium chloride injection.Each group included eight rabbits.One hour after successful modeling,the TA group received an intravitreal injection of 3 mg triamcinolone acetonide,the ART group received an intravitreal injection of the artemether sustained-release preparation,and the Sham and MG groups received an equal volume of 0.9%sodium chloride injection.At six and 24 hours post-LPS injection,anterior segment inflammation was evaluated using slit-lamp bio-microscopy and optical coherence tomography(OCT),and inflammation scores were recorded.Aqueous humor was collected under anesthesia.The number of inflammatory cells was counted using a hemocytometer,and aqueous humor protein concentration was measured using a protein quantification assay kit.RESULTS(1)Anterior segment inflammation:At 6 h post-modeling,all groups exhibited significant inflammatory signs,including conjunctival hyperemia,ciliary congestion,iris vessel dilation,and miosis.At 24 h,inflammation worsened with fibrinous exudation in the anterior chamber,aqueous flare,hypopyon,and pupillary abnormalities.Inflammation scores in the MG group at both 6 h and 24 h were significantly higher than those in the Sham group(P<0.05),while those in the TA and ART groups were significantly lower than in the MG group(P<0.05).There was no significant difference between the TA and ART groups(P>0.05).(2)Aqueous humor cell counts:The MG group showed significantly higher cell counts than the Sham group(P<0.05);The TA and ART groups had significantly lower cell counts than the MG group(P<0.05),with no significant difference between TA and ART groups(P>0.05).(3)Aqueous humor protein concentration:The MG group had significantly higher protein levels compared to the Sham group(P<0.05).Both TA and ART groups exhibited significantly reduced protein concentrations compared to the MG group(P<0.05).However,the ART group had a higher protein concentration than the TA group,with the difference being statistically significant(P<0.05).CONCLUSIONS The artemether sustained-release preparation effectively inhibits anterior segment inflammation in EIU model rabbits.It significantly reduces inflammatory cell infiltration,protein exudation,and clinical signs,demonstrating therapeutic potential for the treatment of acute anterior uveitis.关键词
急性前葡萄膜炎/青蒿琥酯/缓释制剂/炎症反应Key words
acute anterior uveitis/artemether/sustained-release preparation/inflammatory response分类
医药卫生引用本文复制引用
刘亮,郝晓凤,谢立科,孙梅..青蒿琥酯缓释制剂抑制急性前葡萄膜炎模型兔眼前节炎症的实验研究[J].中国中医眼科杂志,2025,35(7):601-606,6.基金项目
1国家自然科学基金项目(82174445)2中国中医科学院眼科医院中央高水平中医医院项目(GSP4-02)3中国中医科学院重大攻关项目(CI2021A05107) （82174445）
