针灸临床杂志2025，Vol.41Issue(6)：69-76,8.DOI:10.19917/j.cnki.1005-0779.025116
基于"肠神并调"探讨针刺对APP/PS1小鼠认知功能的改善机制
Mechanism of Acupuncture in Improving Cognitive Function in APP/PS1 Mice Based on'Intestinal-Spiritual Concomitant Regulation'
摘要
Abstract
Objective:To observe the effects of acupuncture on cognitive function,intestinal mucosal barrier and amyloid β protein(Aβ)in intestine of APP/PS1 mice.Methods:36 male APP/PS1 mice were randomly divided into the model group,the acupuncture group and the probiotic group,with 12 mice in each group.12 male C57BL/6 mice served as the normal control.The acupuncture group was treated with needling Baihui(DU20),Yintang(EX-HN3)and Zusanli(ST36),20 min per time.The probiotics group was treated with probiotics(8.7 × 108 CFU/g).Both groups were intervened once per day for 55 days.Morris water maze was used to observe the behavioral changes.Nissl staining was used to observe the damage of hippocampal neurons.Morphological changes of ileum tissue were observed by HE staining.Immunofluorescence staining was used to observe the expressions of Occludin and Aβ in ileum.The relative expression of Occludin in ileum was examined by Western blotting.Results:Compared with those in the blank group,the escape latency was obviously prolonged(P＜0.01);the number of Nissl staining positive cells in hippocampus was reduced(P＜0.01);the ileal villus height,the ratio of villus height to crypt height,and the expression of Occludin in ileum were markedly decreased(P＜0.01);the expression of Aβ in ileum was significantly increased in the model group(P＜0.01).These changes were reversed by acupuncture and probiotics interventions(P＜0.01,P＜0.05).Conclusion:Needling DU20,EX-HN3 and ST36 can improve the cognitive function of APP/PS1 mice,which may be related to its function in reducing the deposition of Aβ in intestine,alleviating the injury of intestinal mucosal barrier and hippocampal neurons.关键词
阿尔茨海默病/针刺/肠粘膜屏障/β淀粉样蛋白/认知功能Key words
Alzheimer's disease/Acupuncture/Intestinal mucosal barrier/Amyloid β protein/Cognitive function分类
医药卫生引用本文复制引用
郝心,张悦,吴萌,丁宁,李志刚..基于"肠神并调"探讨针刺对APP/PS1小鼠认知功能的改善机制[J].针灸临床杂志,2025,41(6):69-76,8.基金项目
国家自然科学基金,编号:82274654 （）
国家自然科学基金青年项目,编号:82004482 （）
中国中医科学院优秀青年科技人才(创新类)培养专项基金,编号:ZZ14-YQ-012 （创新类）
BAST青年精英科学家资助计划,编号:BYESS2023339. （）
