针灸临床杂志2025，Vol.41Issue(6)：77-82,6.DOI:10.19917/j.cnki.1005-0779.025117
经筋恢刺联合中药熏药治疗对膝骨关节炎模型大鼠NF-κB信号通路的影响
Effect of Meridian Sinew Lateral Needling Combined with Chinese Medical Fumigation on NF-κB Signaling Pathway in KOA Model Rats
摘要
Abstract
Objective:To explore the mechanism of meridian sinew lateral needling combined with Chinese medical fumigation in treatment of knee osteoarthritis(KOA)and its impacts on NF-κB signaling pathway.Methods:The rats were randomly divided into the normal control group(NC),the model(M)group,the Chinese medication control(DZ)group,the meridian sinew lateral needling(HC)group,and the meridian sinew lateral needling combined with Chinese medical fumigation(LH)group.The KOA model was elaborated by using Sodium Iodoacetate.Behavioral scoring was assessed before and after the treatment.Knee joint fluid was collected after the treatment for testing the levels of IL-1 β,IL-6,and TNF-α.Knee joint tissues were collected for histopathological observation.Relative mRNA expressions of IKK NF-κB and p65 were detected by using PCR,and their protein expressions were detected by applying Western blot.Results:The behavioral scores were lower in all treatment groups than that in the M group after the treatment,among which the score in the LH group was lower than that in the DZ group.The levels of IL-1β were decreased in all treatment groups,the level of IL-6 was decreased in the LH group,but the levels of TNF-α did not show significant changes.The levels of IL-1 were significantly reduced in each treatment group,with a significant decrease in the LH group.The tissue damage in each treatment group was reduced,with the LH group showing the best degree of reduction.The relative mRNA expressions of IKK NF-κB and p65,as well as the values of p-IKK/IKK and p-p65/p65 were decreased in each treatment group;among which,the values of p-IKK/IKK and p-p65/p65 in the LH group were lower than those in the DZ group.Conclusion:The regimen of meridian sinew lateral needling combined with Chinese medical fumigation can improve the behavioral score of the affected limbs in the model rats,it can improve joint tissue damage,reduce inflammatory factor levels,and inhibit NF-κB signaling pathway,thus to improve local inflammatory response.关键词
膝骨关节炎/经筋恢刺法/中药熏药/NF-κB信号通路/炎症因子Key words
Knee osteoarthritis/Meridian sinew lateral needling/Chinese medical fumigation/NF-κB signaling pathway/Inflammatory factors分类
医药卫生引用本文复制引用
史孟瑄,史仲珣,袁之鹏,梁卫..经筋恢刺联合中药熏药治疗对膝骨关节炎模型大鼠NF-κB信号通路的影响[J].针灸临床杂志,2025,41(6):77-82,6.基金项目
东部战区空军医院卫勤课题,编号:22YY010. （）
