中国农业科学2025，Vol.58Issue(13)：2487-2503,17.DOI:10.3864/j.issn.0578-1752.2025.13.001
耐低氮小麦品种的筛选及耐低氮指数的全基因组关联分析
Screening of Wheat Varieties with Low Nitrogen Tolerance and Genome-Wide Association Studies of Low Nitrogen Stress Tolerance Index
摘要
Abstract
[Objective]The excessive application of nitrogen fertilizers has led to ecological pollution and waste of agricultural resources.Developing nitrogen-efficient wheat varieties and improving nitrogen use efficiency are effective approaches for achieving sustainable agricultural development and environmental protection.Screening low-nitrogen-tolerant germplasm resources and identifying genetic loci and candidate genes associated with low-nitrogen tolerance can provide materials and theoretical foundations for breeding nitrogen-efficient wheat varieties.[Method]A natural population consisting of 389 wheat varieties was cultivated under high-nitrogen(HN)and low-nitrogen(LN)treatments in 10 field environments.Grain yield per plant(GYP)was measured to calculate the stress tolerance index(STI),thereby enabling the classification of varieties with differential low-nitrogen tolerance.Genome-wide association studies(GWAS)were conducted using 660K SNP array genotyping data to identify stable genetic loci associated with low-nitrogen tolerance.Candidate genes were prioritized through haplotype analysis,expression profiling,and functional annotation.[Result]Twelve wheat varieties with strong low-nitrogen tolerance were identified,including Zhongluo 08-1,Jimai 15,Jinghua 2,Kehong 1,Mianyang 19,Jimai 22,Zhenmai 4,Yumai 35,Fengkang 7,Mianyang 11,Jinmai 31,and Lumai 5.Fourteen loci significantly associated with STI were detected,among which four(qSTI1A.1,qSTI3B,qSTI6A,and qSTI7A.2)overlapped with previously reported low-nitrogen tolerance or yield-related QTLs.Notably,qSTI3B-replicated across three environments-was identified as a key locus governing low-nitrogen tolerance.Functional annotation revealed that its candidate gene,TraesCS3B02G042400,encodes an AP2/EREBP(APETALA2/ethylene-responsive element-binding protein)transcription factor.Haplotype analysis showed significant STI divergence among varieties carrying distinct haplotypes,while expression levels of TraesCS3B02G042400 exhibited nitrogen dose-responsive upregulation.[Conclusion]Twelve wheat varieties with strong low-nitrogen tolerance were screened.A stable genetic locus,qSTI3B,and a candidate gene,TraesCS3B02G042400,associated with low-nitrogen tolerance were identified.关键词
小麦/耐低氮性/耐低氮指数/全基因组关联分析/候选基因Key words
wheat/low nitrogen tolerance/stress tolerance index/genome-wide association study/candidate gene引用本文复制引用
李宁,高丽锋,黄鑫,史华伟,杨进文,史雨刚,陈明,贾继增,孙黛珍..耐低氮小麦品种的筛选及耐低氮指数的全基因组关联分析[J].中国农业科学,2025,58(13):2487-2503,17.基金项目
国家重点研发计划(2022YFD1200201) （2022YFD1200201）
