中国烟草科学2025，Vol.46Issue(3)：12-19,8.DOI:10.13496/j.issn.1007-5119.2025.03.002
烟属40份种质资源的花粉形态及其分类学意义
Pollen Morphology of 40 Germplasm Resources of Nicotiana and Its Taxonomic Significance
摘要
Abstract
To explore the palynological characteristics of Nicotiana and its taxonomic significance,we examined pollen morphology of 36 wild species and 4 cultivated varieties using scanning electron microscopy,and investigated their taxonomic relationships through cluster analysis.The results revealed that all 40 accessions exhibited monad,isopolar pollen grains with tricolporate or tetracolporate apertures,displaying spherical to prolate shapes[P/E=1.66(0.99-2.16)]and measuring 39.96(26.55-58.08)μm×24.28(17.83-33.79)μm.Seven exine ornamentation types were identified,including verrucate,verrucate-rugulate,rugulate,rugulate-striate,striate,striate-reticulate,and reticulate,among which the striate type was the most prevalent,and most species exhibited exine perforations.Pollen size and exine ornamentation were identified as the key diagnostic characteristics for Nicotiana pollen differentiation.Cluster analysis based on characteristic values classified the 40 taxa into three groups at a similarity coefficient of 15.73.The clustering results of six sections(Nicotiana sect.Nicotiana,Nicotiana sect.Paniculatae,Nicotiana sect.Petunioides,Nicotiana sect.Repandae,Nicotiana sect.Tomentosae,and Nicotiana sect.Trigonophyllae)were consistent with the Knapp taxonomic system.The palynological results provide significant corroborative evidence for the taxonomic study of Nicotiana,with the classification results generally supporting findings from molecular systematic research.关键词
烟属/野生种/花粉形态/电镜扫描/分类学Key words
Nicotiana/wild species/pollen morphology/electron microscopy scanning/taxonomy分类
农业科技引用本文复制引用
郭凯阳,王仁刚,王自力,林世锋,杨志晓,张洁,刘国琴..烟属40份种质资源的花粉形态及其分类学意义[J].中国烟草科学,2025,46(3):12-19,8.基金项目
中国烟草总公司贵州省公司科技项目(2023XM04) （2023XM04）
