中国烟草科学2025，Vol.46Issue(3)：20-28,9.DOI:10.13496/j.issn.1007-5119.2025.03.003
与烟草抗黑胫病Ph基因紧密连锁的SNP标记开发
Development of SNP Markers Tightly Linked to the Ph Gene Conferring Resistance to Tobacco Black Shank Disease
摘要
Abstract
Originated from the wild tobacco Nicotiana plumbaginifolia,the Ph gene is a high-quality resistance source to black shank in tobacco breeding.In order to develop SNP markers linked to this gene to meet the demand of current molecular breeding,this study used black shank-resistant flue-cured tobacco variety Coker371-Gold with the Ph gene and a susceptible variety Zhongyantexiang 301 without the Ph gene as materials.Using tobacco SSR markers and SNP variations of 33 cultivated tobacco germplasm resources based on whole genome resequencing,the molecular characteristics of the chromosomal fragment of N.plumbaginifolia carrying the Ph gene was described.By constructing a backcross population segregating for black shank resistance,an integrated genetic map of 8 known molecular markers linked to the Ph gene was drawn,and on the basis,more closely linked SNP markers to the Ph gene were developed.The results showed as the follows.(1)The N.plumbaginifolia chromosomal fragment carrying the Ph gene was probably transferred into cultivated tobacco in the form of terminal translocation,and had a good homology relationship with the replaced cultivated tobacco chromosomal fragment,and its translocation site was located near 18.54 Mb of Yunyan87 HIC_ASM_14 chromosome;(2)Ph gene was located at the end of the translocation fragment,which probably being close to the translocation site,and one closely linked specific SNP marker was obtained on each side of the gene.These results provided a theoretical basis for further creating a more valuable intercalary translocation line with small fragment,thereby providing technical support for better utilization of this black shank disease resistance source in the future.关键词
烟草/黑胫病/Ph基因/易位/单核苷酸多态性Key words
tobacco/black shank/Phgene/translocation/single nucleotide polymorphism分类
农业科技引用本文复制引用
冯慧馨,吴新儒,宗浩,陈梦,刘西金,王大海,吴峰,丁蓬勃,唐利忠,徐展韬,王玉华..与烟草抗黑胫病Ph基因紧密连锁的SNP标记开发[J].中国烟草科学,2025,46(3):20-28,9.基金项目
中国烟草总公司山东省公司科技重点项目(202115、201909) （202115、201909）
中国烟草总公司"揭榜挂帅"项目(110202103009) （110202103009）
