中国农业科学2025，Vol.58Issue(13)：2591-2603,13.DOI:10.3864/j.issn.0578-1752.2025.13.008
油茶壳杀黏虫活性成分鉴定及其作用机制
Insecticidal Active Component Identification of Camellia oleifera Shell Against Mythimna separata and Its Action Mechanism
摘要
Abstract
[Objective]The objective of this study is to clarify the insecticidal active component and mechanism of Camellia oleifera shell on Mythimna separate,and to provide a basis for the development of botanical insecticides for M.separata.[Method]The insecticidal activity of petroleum ether,chloroform,ethyl acetate,and aqueous extracts of C.oleifera shell against 3rd instar larvae of M.separata was evaluated using the immersion and leaf-dipping methods.The petroleum ether extract was subjected to silica gel column chromatography for separation,followed by gas chromatography-mass spectrometry(GC-MS)analysis to identify the active components.Furthermore,a non-targeted metabolomics approach was employed to annotate,screen,and analyze differential metabolites in M.separata.[Result]The petroleum ether extract of C.oleifera shell exhibited the highest contact toxicity against M.separata,with LC50 values of 9.53,8.00,7.51,and 7.02 mg·mL-1 on 1,3,5,and 7 d,respectively.The petroleum ether extract was separated by silica gel column chromatography to obtain 20 fractions,among which fraction 6 showed the best contact toxicity against M.separata,with LC50 values of 3.99,3.85,3.72,and 3.72 mg·mL-1 on 1,3,5,and 7 d,respectively.Further separation of fraction 6 yielded 21 subfractions,with subfraction 6.4 demonstrating the highest contact toxicity.GC-MS analysis identified hexadecane(19.33%),1-hexadecene(14.21%),octacosane(4.03%),octadecane(9.24%),dichlorochrysanthemic acid(6.73%),1-octadecene(7.27%),1-tetracosanol(3.87%)and isophytol(5.92%)as the main compounds.Among these,dichlorochrysanthemic acid exhibited the strongest contact toxicity against M.separata,with LC50 values of 91.62,55.61,34.94,and 24.43 μg·mL-1 on 1,3,5,and 7 d,respectively.Its insecticidal efficacy was comparable to that of the positive control,pyrethrin.Non-targeted metabolomics analysis identified six differential metabolites,among which piceid,cannabinoid,5-fluoro-cannabinoid metabolite,and caproylglycine were significantly upregulated,while 4-ethyl-2,6-dihydroxyphenyl sulfate and N-pentacosanoylglycine were significantly downregulated.These differential metabolites were primarily enriched in four metabolic pathways:cofactor biosynthesis,tryptophan metabolism,niacin and nicotinamide metabolism,ubiquinone and terpenoid-quinone biosynthesis.[Conclusion]Dichlorochrysanthemic acid from C.oleifera shell extract exhibits insecticidal activity against M.separata,providing a new avenue for the biological control of M.separata and the high-value utilization of C.oleifera shell.关键词
油茶壳/黏虫/触杀活性/二氯菊酸/代谢组学Key words
Camellia oleifera shell/Mythimna separata/contact toxicity/dichlorochrysanthemic acid/metabolomics引用本文复制引用
詹丽,梁宗锁,于靖,卢俊,梁倩..油茶壳杀黏虫活性成分鉴定及其作用机制[J].中国农业科学,2025,58(13):2591-2603,13.基金项目
云南省教育厅科学研究基金(2024Y578)、浙江省尖兵领雁研发攻关计划(2022C02023)、云南省农业基础研究联合专项面上项目(202401BD070001-105) （2024Y578）
