中国农业科学2025，Vol.58Issue(13)：2604-2613,10.DOI:10.3864/j.issn.0578-1752.2025.13.009
不同植稻年限土壤酶活性及其化学计量特征
Soil Enzyme Activities and Their Stoichiometry Under Prolonged Rice Cultivation
摘要
Abstract
[Objective]The theoretical evaluation of ecological stoichiometry of soil microbial metabolism and soil nutrient restriction in paddy soil was carried out,so as to provide the support for understanding the nutrient cycle of rice ecosystem.[Method]In this study,a chronosequence of paddy soils with 1 000 years was collected and analyzed soil enzyme activity and enzyme stoichiometry ratio with prolonged rice cultivation and clarified the influencing factors.[Result]The invertase activity increased with the chronosequence,while the activities of α-1,4-glucosidase,β-1,4-glucosidase,β-D-cellobiohydrolase and β-1,4-xylosidase enzymes decreased significantly at the early stage of chronosequence(50 years),and then increased significantly and remained stable.The dynamic of urease was similar to invertase,while the activities of n-acetyl-β-glucosaminidase were similar to most of the enzymes concerned in the carbon cycle.The C:N ratio of soil enzymes was the lowest at 1.28 when the soil was not cultivated(0 year),while the C:P and N:P ratios of soil enzymes were the highest at 1.77 and 1.38 at the same time,respectively.Generally,the C:N and C:P ratios of soil enzymes were all greater than 1 in all rice-growing years,while the N﹕P ratio of soil enzymes was less than 1 except for the uncultivated soil(0 year).[Conclusion]According to the comprehensive stoichiometric analysis(soil enzyme activity ratio,vector length and angle)could be seen that the soil microorganisms in the marsh before reclamation were mainly limited by carbon,while the soil microorganisms in the early stage(50 years)of rice cultivation after reclamation were mainly limited by phosphorus,and with the chronosequence,the soil microorganisms were changed to be limited by nitrogen.The research results could provide a theoretical basis for understanding the biogeochemical cycling mechanisms mediated by microorganisms in paddy ecosystems,as well as guiding soil nutrient management and ecological sustainable development.关键词
土壤酶/酶化学计量/水稻土/时间序列/土壤发育Key words
soil enzyme/enzyme stoichiometry/paddy soil/soil chronosequence/soil development引用本文复制引用
张鑫瑶,王萍,刘亚龙,汪景宽..不同植稻年限土壤酶活性及其化学计量特征[J].中国农业科学,2025,58(13):2604-2613,10.基金项目
辽宁省教育厅基本科研项目(JYTYB2024009) （JYTYB2024009）
