中国烟草科学2025，Vol.46Issue(3)：70-80,11.DOI:10.13496/j.issn.1007-5119.2025.03.009
本氏烟tsRNAs对马铃薯Y病毒侵染的响应
Altered Expression of Transfer-RNA-derived small RNAs after Potato Virus Y Infection in Nicotiana benthamiana
摘要
Abstract
To explore the molecular mechanisms underlying Nicotiana benthamiana responses to potato virus Y(PVY)infection,small RNA sequencing(sRNA-seq)and transcriptome sequencing(RNA-seq)were employed to analyze the differences in tsRNAs and transcriptional responses in N.benthamiana following PVY infection.The results showed that 39 differentially expressed tiRNAs(tRNA halves)and 119 tRFs(tRNA-related fragments)were identified at the 7th day post-inoculation.Enrichment analysis of differentially expressed target genes(DETGs)demonstrated that 117 and 602 target genes of tRFs in leaves and stems,respectively,were predominantly enriched in pathways such as signal transduction and energy metabolism.Notably,the expression of tDR-1:18-His-GTG-1-M3 was most significantly down-regulated in PVY-infected leaves.Suppressing the expression of tDR-1:18-His-GTG-1-M3 in N.benthamiana markedly increasing the expression levels of its target genes,yabD and RNF170.These findings provide a reference for further in-depth research on the role of tsRNAs in PVY-host plant interactions and the development of PVY control strategies.关键词
本氏烟/马铃薯Y病毒/小RNA测序/转录组测序Key words
Nicotiana benthamiana/potato virus Y/small RNA sequencing/transcriptome sequencing分类
农业科技引用本文复制引用
高鑫文,宋洪平,王惠,苗圃,焦裕冰,申莉莉,王玉洁,杨金广..本氏烟tsRNAs对马铃薯Y病毒侵染的响应[J].中国烟草科学,2025,46(3):70-80,11.基金项目
青岛市科技惠民示范专项(23-2-8-xdny-12-nsh) （23-2-8-xdny-12-nsh）
河南省烟草公司洛阳市公司科技项目(2023410300200041) （2023410300200041）
