安徽农业大学学报2025，Vol.52Issue(3)：414-419,6.DOI:10.13610/j.cnki.1672-352x.20250704.012
玉米ZmUBP18基因调控盐胁迫机制研究
Mechanism of ZmUBP18 gene in regulation of salt tolerance in maize
摘要
Abstract
[Objective]The study aimed to analyze the physicochemical and molecular properties of the maize ubiquitin specific protein ZmUBP18,and further elucidate the biological mechanism by which ZmUBP18 regulates maize response to salt stress.[Method]Through homology analysis,it was found that the ZmUBP18 gene may be involved in the abiotic stress response pathway of maize.Subsequently,the zmubp18 mutant from the maize mutant library was identified,the related phenotypes of zmubp18 under salt stress was analyzed,and the main physiological indicators of stress response in zmubp18,as well as the transcriptomic differences between zmubp18 and the wild-type under salt stress were analyzed.[Result]Under salt stress,the root length of zmubp18 mutants was significantly shorter compared to the wild type,exhibiting a hypersensitive phenotype,indicating that ZmUBP18 is involved in maize salt stress signaling.Physiological data revealed de-creased activities of SOD and POD,along with increased MDA content in zmubp18 under salt stress,suggest-ing severe membrane system damage and reduced salt tolerance.Transcriptomic analysis showed that salt stress-induced upregulated genes were significantly reduced in zmubp18,implying that the mutation suppresses maize salt stress response,leading to hypersensitivity.Signaling pathway analysis further demonstrated weak-ened enrichment of defense-and stress response-related genes in zmubp18 compared to the wild type under salt stress.[Conclusion]ZmUBP18 positively regulates the tolerance of maize in response to salt stress.关键词
玉米/ZmUBP18基因/盐胁迫/根系发育Key words
maize/ZmUBP18/salt stress/root development分类
农业科技引用本文复制引用
谢丹丹,苗良杰,王梦瑶,朱文艺,程莹,江丽斌,彭元成..玉米ZmUBP18基因调控盐胁迫机制研究[J].安徽农业大学学报,2025,52(3):414-419,6.基金项目
国家自然科学基金面上项目(32071923)资助. （32071923）
