安徽农业科学2025，Vol.53Issue(14)：9-15,7.DOI:10.3969/j.issn.0517-6611.2025.14.002
一株水稻促生贝莱斯芽孢杆菌20-10的高密度液体发酵技术研究
Study on High Density Liquid Fermentation Technology of a Rice Growth Promoting Bacillus velezensis 20-10
摘要
Abstract
The single factor and orthogonal experiments were used to optimize the composition and content of liquid culture medium for Bacil-lus velezensis 20-10,the optimal culture medium components were maltose 20.0 g/L,starch 10.0 g/L,yeast paste 12.5 g/L,NaCl 1.0 g/L,(NH4)2 SO4 3.0 g/L.In this culture medium,the bacteria concentration was 45.5×108 CFU/mL,which increased by 106.82%compared to the basic culture medium,after being cultured in strain 20-10 shake flasks for 72 hours.The optimal fermentation parameters obtained by opti-mizing the fermentation conditions of 20-10 through single factor and response surface experiments were inoculation amount 2.0%,temperature 30℃,and pH 7.0.Under these optimized conditions,the bacterial concentrations obtained by expanding the cultivation of the strains in 20 L and 500 L fermenters for 64 hours were 52.0×108 and 160.5×108 CFU/mL,respectively,which were 1.36 and 6.30 times higher than the bas-ic culture medium.关键词
贝莱斯芽孢杆菌20-10/正交试验/响应面试验/高密度液体发酵/发酵条件优化Key words
Bacillus velezensis 20-10/Orthogonal experiment/Response surface experiment/High density liquid fermentation/Fermentation op-timization分类
农业科技引用本文复制引用
杨华,胡展,伍善东,肖蓉,谭石勇,刘朱东,程伟,郭照辉,付祖姣..一株水稻促生贝莱斯芽孢杆菌20-10的高密度液体发酵技术研究[J].安徽农业科学,2025,53(14):9-15,7.基金项目
湖南省技术攻关"揭榜挂帅"项目(2021NK1040) （2021NK1040）
湖南省自然科学基金项目(2021JJ30411). （2021JJ30411）
