北京中医药大学学报2025，Vol.48Issue(7)：933-942,10.DOI:10.3969/j.issn.1006-2157.2025.07.007
益气解毒方对电离辐射诱导的巨噬细胞极化的调控作用及机制研究
Regulatory effect and mechanism of Yiqi Jiedu Decoction on ionizing radiation-induced macrophage polarization
摘要
Abstract
Objective To investigate the regulatory effect and mechanism of Yiqi Jiedu Decoction(YQJD)on ionizing radiation-induced macrophage polarization and its correlation with the Toll-like receptor 4(TLR4)/myeloid differentiation primary response protein 88(MyD88)/nuclear factor kappa-B(NF-κB)signaling pathway.Methods Fifty-five specific-pathogen-free male Sprague-Dawley rats were randomly divided into blank(n=30),anduolin(n=10),and YQJD groups(n=15).They were respectively gavaged with deionized water,anduolin suspension(0.345 6 g/kg),and YQJD high-dose(20.88 g/kg)at a dose of 0.01 mL/g body weight once a day for seven consecutive days.2 hours after the last gavage,blood was collected from the abdominal aorta to prepare the control rat,andolin rat,and YQJD high-dose sera.Appropriate amounts of YQJD high-dose and control sera were mixed in a ratio of 1∶1 and 1∶3,respectively,to obtain YQJD medium-and low-dose rat serum.RAW264.7 cells were divided into blank(10%blank rat serum),model(10%blank rat serum),anduolin(10%anduolin rat serum),and YQJD-L,YQJD-M,YQJD-H groups(10%YQJD low-,medium-,and high-dose rat serum).Except for the blank group,the cells in other groups were irradiated with 12 Gy60 Co γ-rays once to establish the macrophage radiation injury model.At 24 h after irradiation,cell viability was detected using the CCK-8 method,and the cell migration rate was measured using the scratch test.Cell morphology was observed using phalloidin staining,tumor necrosis factor-alpha(TNF-α)and interleukin-10(IL-10)levels in the cell supernatant were quantified using enzyme-linked immunosorbent assay,and the proportion of M1 macrophages was detected using flow cytometry.TLR4,MyD88,and NF-κB protein expression were detected using Western blotting.Results Twenty-four hours after irradiation,compared with the blank group,the model group exhibited significantly reduced cell viability and migration rate(P<0.01),increased cell volume and pseudopodia formation,elevated TNF-α and IL-10 levels,an increased proportion of M1 macrophages,and upregulated TLR4,MyD88,and NF-κB protein expression(P<0.05,P<0.01).Compared with the model group,each drug-treated group showed improved cell viability and migration rate(P<0.05,P<0.01),decreased cell volume,more regular cell shape,reduced TNF-α levels,lower M1-type macrophage proportion,and downregulated TLR4,MyD88,and NF-κB protein expression(P<0.05,P<0.01).IL-10 level showed an upward trend.Conclusion YQJD can partially inhibit M1 macrophage polarization and suppress inflammatory responses,which may be related to the TLR4/MyD88/NF-κB signaling pathway.关键词
益气解毒方/电离辐射/巨噬细胞极化/Toll样受体 4/髓样分化初级反应蛋白质 88/核因子-κB信号通路/大鼠Key words
Yiqi Jiedu Decoction/ionizing radiation/macrophage polarization/Toll-like receptor 4/myeloid differentiation primary response protein 88/nuclear factor kappa-B pathway/rats分类
医药卫生引用本文复制引用
胡瑞瑶,赵章弟,王安,李文元,雷家俊,曾嘉欢,安梓睿,胡素敏..益气解毒方对电离辐射诱导的巨噬细胞极化的调控作用及机制研究[J].北京中医药大学学报,2025,48(7):933-942,10.基金项目
国家自然科学基金面上项目(No.12075035) （No.12075035）
2023 年北京中医药大学本博贯通学生科学研究项目(No.XBB23035) National Natural Science Foundation of China(No.12075035) （No.XBB23035）
