草业科学2025，Vol.42Issue(6)：1331-1342,12.DOI:10.11829/j.issn.1001-0629.2024-0480
基于宏转录组的宁夏荒漠灌丛根际土壤微生物群落组成和功能多样性
Macrotranscriptome-based rhizosphere soil microbial community composition and functional diversity in the desert shrublands of Ningxia
摘要
Abstract
Metatranscriptomic sequencing analysis was conducted on four typical legume shrub rhizosphere soil samples from the Ningxia Baijitan Desert Nature Reserve to investigate the composition of functional microbial communities and the expression characteristics of functional genes in the rhizosphere soil of desert shrubs,and to explore potential stress-resistant and growth-promoting functional genes.The results demonstrated significant differences in active bacterial communities in the rhizosphere soil of the four shrubs.The relative abundance of Actinobacteria,Chloroflexi,and Acidobacteria in the rhizosphere soil of Caragana intermedia,Ammopiptanthus mongolicus,and Oxytropis aciphylla was significantly higher than that of C.tibetica.In comparison,the relative abundance of the Proteobacteria,Pseudomonas,was significantly lower than that of C.tibetica.The functional annotation of the Cluster of Orthologous Groups of Proteins indicated that the functional pathways with significant differences among groups included energy generation and conversion,cytoskeleton,etc.The results of the Kyoto Encyclopedia of Genes and Genomes Functional Notes demonstrated that the relative abundance of genes related to amino acid metabolism,coenzyme metabolism,and vitamin metabolism pathways in the rhizosphere soil of C.tibetica was significantly higher than those of the other three shrub soils.The transcriptional activities of proX and proW genes encoding osmoregulatory substances,kdpB and NhaA genes encoding reverse transport system,iaaM,and kynU genes encoding indoleacetic acid(IAA)synthesis,and gcd genes encoding phosphorus solubilization were significantly higher in C.tibetica rhizosphere soil than those of the other three soils.Redundancy analysis showed that Actinobacteria and Proteobacteria were positively correlated with total nitrogen(TN)and available phosphorus(AP),while Chloroflexi were significantly negatively correlated with soil organic matter(SOM),total nitrogen(TN),total phosphorus(TP),and available potassium(AK).The abundance of functional genes involved in transcription and nucleic acid metabolism was positively correlated with TP,and the abundance of genes involved in xenobiotic biodegradation and metabolism was positively correlated with TN.The result can provide a theoretical basis for further exploration of the relationship between the structure and function of soil microbial communities in specific desertification habitats and lay a foundation for enriching microbial and gene resources in desert areas.关键词
荒漠植物/根际土壤/细菌群落/转录活性/功能途径/抗逆基因/促生基因Key words
desert plants/rhizosphere soil/bacterial community/transcriptional activity/functional pathways/stress resistance gene/promote growth gene引用本文复制引用
姚佳妮,代金霞,刘爽,张钧杰,胡明珠..基于宏转录组的宁夏荒漠灌丛根际土壤微生物群落组成和功能多样性[J].草业科学,2025,42(6):1331-1342,12.基金项目
国家自然科学基金项目(32160023) （32160023）
宁夏自然科学基金项目(2023AAC03026) （2023AAC03026）
