草业科学2025，Vol.42Issue(6)：1343-1353,11.DOI:10.11829/j.issn.1001-0629.2024-0536
小果白刺AQP家族鉴定及生物信息学分析
Identification and bioinformatic analysis of the AQP family in Nitraria sibirica
摘要
Abstract
Nitraria sibirica,a typical halophytic shrub belonging to the family Nitrariaceae within the order Sapindales,is primarily distributed in desert regions and inland arid areas of China,such as the Hexi Corridor.This plant exhibits remarkable tolerance to abiotic stresses,including drought and salinity.It is frequently utilized as a pioneer species in grassland ecological restoration and the reclamation of heavily saline-alkali soils.Aquaporins(AQPs)are a class of membrane-integrated proteins that regulate the transmembrane transport of water and other small neutral molecules by forming channels across cell membranes,playing a crucial role in plant stress responses.However,the biological functions of the AQP family in the response of N.sibirica to salt stress have not yet been reported.In this study,we explored the salt tolerance mechanisms of N.sibirica by conducting the first genome-wide systematic identification of the NsAQP gene family based on genomic and transcriptomic data.A total of 24 NsAQP genes were identified,with subcellular localization predictions indicating their predominant distribution in the plasma membrane and vacuolar membrane.Through phylogenetic analysis,these genes were classified into five subfamilies:PIP,TIP,NIP,SIP,and XIP,with members within the same subfamily sharing similar conserved motifs and gene structures.Promoter cis-acting element analysis revealed that the NsAQP gene family contains various regulatory elements associated with abiotic stress responses.Analysis of transcriptomic expression patterns under salt stress further demonstrated differential expression profiles of NsAQP genes across different tissues and organs,suggesting their potential involvement in salt stress responses through the regulation of water transport.This study provides a significant theoretical foundation and genetic resources for elucidating the salt tolerance mechanisms of N.sibirica,while also offering new approaches for improving plant stress resistance.关键词
NsAQP/盐胁迫/水通道蛋白/系统进化分析/理化性质/基因结构/基因表达水平Key words
NsAQP/salt stress/aquaporins/phylogenetic analysis/physicochemical properties/gene structure/gene expression level引用本文复制引用
魏娜,许尔文,朱希玉,吕东,王顺利,王艳霞,刘红梅,赵祜,陈刚,赵晶忠..小果白刺AQP家族鉴定及生物信息学分析[J].草业科学,2025,42(6):1343-1353,11.基金项目
甘肃省科技计划项目(23YFFG0002) （23YFFG0002）
甘肃省省级人才(青年团队)项目(甘组通字[2024]4号) （青年团队）
张掖市市级科技计划项目(ZY2023KY02、ZY2024KY01) （ZY2023KY02、ZY2024KY01）
甘肃省科技重大专项计划—国际合作领域项目(25ZDWA007) （25ZDWA007）
