草业科学2025，Vol.42Issue(6)：1414-1427,14.DOI:10.11829/j.issn.1001-0629.2023-0491
不同氮浓度条件下内生真菌对醉马草生长和氮代谢的影响
Effects of endophytic fungi on the growth and nitrogen metabolism of Achnatherum inebrians under different nitrogen concentrations
摘要
Abstract
The endophytic fungi Epichloë gansuensis can influence the growth and nitrogen metabolism of the host plant Achnatherum inebrians through symbiotic association,with these effects being closely related to environmental nitrogen concentrations.Current research remains insufficient regarding the symbiotic interactions between E.gansuensis and A.inebrians under elevated nitrogen conditions.To investigate the impact of endophyte infection on A.inebrians growth and nitrogen metabolism under high nitrogen concentrations,we conducted a pot experiment comparing endophyte-infected(E+)and endophyte-free(E-)plants under 15 and 30 mmol·L-1 nitrogen applications.Post-cultivation measurements included dry biomass,inorganic nitrogen accumulation,total nitrogen content,key nitrogen metabolism enzyme activities,and chlorophyll levels.Results demonstrated that at 15 mmol·L-1 nitrogen,E+plants exhibited 78.99％significantly higher dry weight than E-plants(P＜0.05),with leaf ammonium nitrogen content being significantly lower at 12.27％.Under 30 mmol·L-1 nitrogen,root nitrate and ammonium nitrogen contents in E+plants were 15.56％and 18.78％,respectively,significantly lower than their E-counterparts.E.gansuensis enhanced photosynthetic capacity and nitrogen utilization efficiency in E+plants under high nitrogen conditions,optimized nitrogen allocation patterns,and significantly reduced inorganic nitrogen accumulation(P＜0.05),collectively improving host adaptation to nitrogen-rich environments.This study provides theoretical insights into the ecological functions of E.gansuensis-A.inebrians symbiosis and potential agricultural applications of grass-endophyte systems in high-nitrogen habitats.关键词
内生真菌/醉马草/氮代谢/共生效应/氮利用效率/氮分配策略Key words
endophytic fungi/Achnatherum inebrians/nitrogen metabolism/symbiotic effects/nitrogen use efficiency/nitrogen allocation strategy引用本文复制引用
李星陆,郑蓉,邓茂桦,王蓓晨,刘荣贵,王剑峰..不同氮浓度条件下内生真菌对醉马草生长和氮代谢的影响[J].草业科学,2025,42(6):1414-1427,14.基金项目
国家自然科学基金项目(32001399) （32001399）
