长春中医药大学学报2025，Vol.41Issue(7)：750-755,6.DOI:10.13463/j.cnki.cczyy.2025.07.010
苓泽合剂对痛风性关节炎大鼠模型MMP-3表达的影响
Effect of Lingze Mixture on the expression of matrix metalloproteinase-3 in rats model with gouty arthritis
摘要
Abstract
Objective To establish a rat model of gouty arthritis(GA)and to study the therapeutic and protective effects of Lingze mixture on the articular cartilage of rats with acute gouty arthritis by observing the pathological morphological changes and detecting the expression levels of matrix metalloproteinase-3(MMP-3)in serum and cartilage.Methods A total of 70 rats were randomly divided into the blank group,the model group,the etoricoxib group,the gout group,and the Lingze mixture low dose group,the Lingze mixture medium dose group and the Lingze mixture high dose group,with 10 rats in each group.Except for the blank group,intra-articular injection of sodium urate combined with intraperitoneal injection of potassium oxazinate was used to induce rat GA model.After successful modeling,the administration groups were orally administered according to the prescribed dose,and the model group and the blank group were given the same volume of distilled water,once a day for 3 consecutive days.After successful modeling,the serum of rats was taken to detect the content of MMP-3 in the blood.The cartilage of the right ankle joint of rats was taken to observe the pathological morphological changes,and the expression of MMP-3 in the cartilage was detected by immunocytochemistry.Results After administration,the destruction of articular cartilage in the Lingze mixture low dose group,the Lingze mixture medium dose group and the Lingze mixture high dose group was significantly reduced compared with the model group;The content of MMP-3 in the articular cartilage in the model group was significantly increased,the content of MMP-3 in articular cartilage was decreased in both Lingze mixture group and the positive drug group;and the expression of MMP-3 in articular cartilage in the Lingze mixture was decreased group,the Lingze mixture high dose group and etoricoxib group was better than that in the gout group and Lingze mixture low dose group.The serum concentration of MMP-3 in the Lingze mixture low dose group was significantly lower than that in the model group(P<0.05).The serum concentration of MMP-3 in the Lingze mixture medium dose group and the Lingze mixture high dose group was csignificantly lower than that in the model group(P<0.01).Conclusion Lingze mixture may treat and prevent the destruction of articular cartilage in gouty arthritis by inhibiting the expression of MMP-3 in blood.关键词
苓泽合剂/痛风性关节炎/基质金属蛋白酶-3Key words
Lingze mixture/gouty arthritis/matrix metallo proteinases-3分类
医药卫生引用本文复制引用
高志鹏,赵用,孔德花,马宝东,陈岩松,甘雨..苓泽合剂对痛风性关节炎大鼠模型MMP-3表达的影响[J].长春中医药大学学报,2025,41(7):750-755,6.基金项目
辽宁省科学技术计划项目(2022JH2/101300047) （2022JH2/101300047）
沈阳市科学技术计划项目(24-214-3-180) （24-214-3-180）
第五批全国中医临床优秀人才项目(国中医药人教函[2022]1号) （国中医药人教函[2022]1号）
第一批辽宁省中医临床优秀人才项目(辽卫发[2021]7号) （辽卫发[2021]7号）
辽宁省闫也名中医工作室建设项目(2024) （2024）
