草原与草坪2025，Vol.45Issue(3)：60-69,10.DOI:10.13817/j.cnki.cyycp.2025.03.007
桃色欧文氏菌Cp2粉红色素对紫花苜蓿幼苗可培养细菌群落结构的影响
Effect of Erwinia persicina Cp2 pink pigment on al-falfa seedlings in culturable bacterial community structure
摘要
Abstract
[Objective]To explore the effect of Erwinia persicina Cp2 pink pigment on the structure of cultur-able bacterial communities in alfalfa seedlings.[Method]In this experiment,alfalfa'Juneng 551'was treated with different concentrations of Cp2 pink pigment(0,0.094,0.188,0.282,0.376,0.470 mg/mL).After 21 days of growth,the aboveground and underground culturable bacteria of alfalfa seedlings were isolated and purified.[Result]1)After 16S rRNA sequencing,a total of 15 strains of bacteria were isolated from alfalfa seedlings,belonging to 3 phyla and 11 genus.The dominant phylum was Firmicutes,accounting for 40％of the phylum level,and the dominant genus was Bacillus,accounting for 20％of the genus level.2)Cp2 pink pigment can significantly increase the number of aboveground and underground culturable bacteria in alfalfa seedlings compared with CK,and the number of under-ground culturable bacteria in alfalfa seedlings is higher than that above ground.3)Cp2 pink pigment altered the domi-nant bacterial genera within the culturable community of alfalfa seedlings.With the increase of Cp2 pink pigment con-centration,the dominant genus shifted progressively from Bacillus to Brevibacillus,then to Leclercia,and ultimately to Pantoea.[Conclusion]Cp2 pink pigment of Erwinia persicina changes the community structure of culturable bac-teria in alfalfa seedlings.关键词
紫花苜蓿/可培养细菌/粉红色素/桃色欧文氏菌/群落结构Key words
alfalfa/culturable bacteria/pink pigment/Erwinia persicina/community structure分类
农业科技引用本文复制引用
黄荣,田望军,陈海雁,杨杰,谢金晶,张振粉..桃色欧文氏菌Cp2粉红色素对紫花苜蓿幼苗可培养细菌群落结构的影响[J].草原与草坪,2025,45(3):60-69,10.基金项目
国家自然科学基金项目(32060369) （32060369）
甘肃省杰出青年基金项目(20JR10RA562) （20JR10RA562）
草业生态系统教育部重点实验室—人才培育项目(KLGE202203) （KLGE202203）
国家现代农业产业技术体系(CARS-07-C-1) （CARS-07-C-1）
