草原与草坪2025，Vol.45Issue(3)：90-99,10.DOI:10.13817/j.cnki.cyycp.2025.03.010
罗布麻COMT基因家族的生物信息学分析
Bioinformatics analysis of COMT gene family of Apocynum venetum
摘要
Abstract
[Objective]COMT gene family members play important regulatory roles in plant lignin synthesis,growth,development and abiotic stress.The study was carried out to analyze the structure,expression,protein physi-cochemical properties,and function of the AvCOMT gene in lignin biosynthesis of Apocynum venetum.[Method]The chromosome location,protein structure,and physicochemical properties,homeostatic elements,and phylogenetic tree were predicted and analyzed by bioinformatics methods.[Result]The results showed that there were 10 mem-bers of the COMT gene family in A.venetum,distributed on 3 chromosomes,including 1-4 exons and 3-10 con-served domains.Its secondary structure was mainly composed of α—helix and random coil.The tertiary structures of AvCOMT01,AvCOMT02,AvCOMT03,AvCOMT05,and AvCOMT08 proteins were similar to that of Arabidop-sis thaliana AtCOMT protein.Among the 10 COMT proteins,AvCOMT07 and AvCOMT10 were unstable pro-teins,and the remaining members were stable proteins.AvCOMT01,AvCOMT03,and AvCOMT04 were hydro-phobic proteins,and the rest were hydrophilic proteins.The expression of AvCOMT gene in A.venetum was regu-lated by environmental factors such as light and temperature,as well as plant hormones such as auxin,gibberellin,and abscisic acid.[Conclusion]A total of 10 AvCOMT genes have been screened from A.venetum.They are similar to the structure and function of A.thaliana AtCOMT and play a key role in plant lignin synthesis and stress resistance.关键词
罗布麻/COMT基因家族/木质素/生物信息学分析/抗逆性/生长发育Key words
Apocynum venetum/COMT gene family/lignin/bioinformatics analysis/resistance/growth and de-velopment分类
农业科技引用本文复制引用
赵长海,李国旗,车金凤..罗布麻COMT基因家族的生物信息学分析[J].草原与草坪,2025,45(3):90-99,10.基金项目
宁夏自然科学基金项目(2020AAC03076) （2020AAC03076）
宁夏重点研发项目(2022BEG03066) （2022BEG03066）
