大豆科学2025，Vol.44Issue(4)：12-19,8.DOI:10.11861/j.issn.1000-9841.2025.04.0012
大豆GmNAC46转录因子的亚细胞定位及形成同源二聚体分析
Analysis of Subcellular Localization and Homodimer Formation of Soybean GmNAC46 Transcription Factors
摘要
Abstract
NAC transcription factors play an important role in plant defending against stress and growth and development.GmNAC46 negatively regulates soybean response to salt stress.In order to analyze the mechanism of action of GmNAC46 transcription factors,the subcellular localization of GmNAC46 transcription factors and whether GmNAC46 can interact with each other to form homodimers were analyzed using bioinformatics methods,tobacco transient expression systems,and yeast two hybrid methods.The bioinformatics prediction results indicate that GmNAC46 is localized in the nucleus and has a homodimeric structure,and a GmNAC46-pEGAD vector was constructed.The transient expression results indicate that GmNAC46 is localized in the nucleus of tobacco epidermal cells,suggesting that GmNAC46 is a nuclear localized transcription factor.We construct GmNAC46-N-pGBKT7 and GmNAC46-pGADT7 vectors,yeast two hybrid results further indicate that GmNAC46 protein can function in cells in the form of homodimers.This research provides a theoretical basis for further exploring the biological function and mechanism of action of soybean GmNAC46 transcription factor.关键词
大豆/GmNAC46/亚细胞定位/同源二聚体/烟草瞬时表达/酵母双杂交Key words
soybeans/GmNAC46/subcellular localization/homodimer/tobacco transient expression/yeast two-hybrid引用本文复制引用
孙睿加,高歌,倪志勇,于月华..大豆GmNAC46转录因子的亚细胞定位及形成同源二聚体分析[J].大豆科学,2025,44(4):12-19,8.基金项目
新疆维吾尔自治区自然科学基金(2022D01E14) （2022D01E14）
国家自然科学基金(32360454) （32360454）
天山英才培养计划(2023TSYCCX0012). （2023TSYCCX0012）
