大豆科学2025，Vol.44Issue(4)：35-47,13.DOI:10.11861/j.issn.1000-9841.2025.04.0035
大豆GmHORSTs基因生物信息学分析、盐胁迫响应表达及亚细胞定位研究
Bioinformatics Analysis,Salt Stress-Responsive Expression,and Subcellular Localization of GmHORSTs Genes in Soybean
摘要
Abstract
CYP86A subfamily genes are involved in the regulation of plant growth and development,especially in the process of plant response to stress.This study identified the homologous gene GmHORSTs of AtHORST/AtCYP86A1 in soybean by homology comparison.Subsequently,bioinformatics methods were used to comprehensively analyzed the gene structure and cis-acting elements in the promoter region of GmHORSTs,as well as the physicochemical properties and secondary structure of the proteins they encode.The tissue expression pattern of GmHORSTs and the expression changes under NaCl stress were analyzed by qRT-PCR.The subcellular localization of the protein encoded by GmHORSTs in tobacco was analyzed by transient transfection technology.The results showed that soybean CYP86A subfamily genes have eight members,and GmHORSTa(Glyma.11G175900)and GmHORSTb(Glyma.14G192500)are homologous genes of AtHORST/AtCYP86A1 in soybean.The gene structure of GmHORSTa and GmHORSTb is highly similar,the proteins encoded by the genes have the characteristic structural loci of CYP450 protein,and the promoter sequences of the two genes contain a variety of cis-acting elements related to growth and development,hormone response and stress response,and the physicochemical properties of the proteins are obviously different,but the secondary structural components are the same and both are transmembrane proteins without signal peptides.The qRT-PCR analysis revealed that GmHORSTa and GmHORSTb are root-specifically expressed genes and are upregulated by NaCl stress induction.Subcellular localization results demonstrated that both GmHORSTa and GmHORSTb proteins are localized to the endoplasmic reticulum.This study provides a reference for further analyzing the function of GmHORSTs in the process of salt stress response.关键词
大豆/CYP86A1/盐胁迫/表达分析/亚细胞定位Key words
soybean/CYP86A1/salt stress/expression analysis/subcellular localization引用本文复制引用
李赵博,厉书媛,周筱钰,元明浩,姜龙,刘明,赵俊淞,闻馨月..大豆GmHORSTs基因生物信息学分析、盐胁迫响应表达及亚细胞定位研究[J].大豆科学,2025,44(4):35-47,13.基金项目
吉林省科技发展计划项目(YDZJ202301ZYTS359). （YDZJ202301ZYTS359）
