动物医学进展2025，Vol.46Issue(8)：1-6,6.
结肠弯曲菌glyA基因质粒DNA标准物质的研制
Development of Plasmid DNA Standards for Campylobacter coli glya Gene
摘要
Abstract
This study was conducted to develop Campylobacter coli standards using droplet digital PCR(ddPCR)detection method.The glyA gene was used as the target gene to establish the ddPCR method,and the specificity,reproducibility and sensitivity of the method were tested and clinically applied.Candidate standards containing the Campylobacter glyA gene were prepared,and the established ddPCR method was applied to evaluate the homogeneity and stability of the standards,and the values were jointly determined by nine laboratories.The established dd PCR method has high specificity,good reproducibility and high sen-sitivity;the prepared standard substance has good homogeneity and can be stably stored for 12 months at-20℃,and the result of the calibration was 5202.60 copies/μL with an uncertainty of 0.43 × 103 copies/μL.The development of this standard substance provides the molecular biology detection method of Campy-lobacter colonis,and provides the support of metrological traceability and quality control for the molecular biology detection method of Campylobacter coli,and helps to promote the standardisation of related detec-tion reagents.关键词
结肠弯曲菌/glyA基因/标准物质/微滴式数字PCR/应用Key words
Campylobacter coli/glyA gene/Standard material/Microtitre digital PCR/Application分类
农业科技引用本文复制引用
李昕,王君玮,邹明,张喜悦,徐佳微,刘意,赵格,曲志娜,王琳,赵建梅,王娟..结肠弯曲菌glyA基因质粒DNA标准物质的研制[J].动物医学进展,2025,46(8):1-6,6.基金项目
国家重点研发计划项目(2022YFC2303900,2022YFD1301003) （2022YFC2303900,2022YFD1301003）
中国动物卫生与流行病学中心创新基金项目(DW2021001-13) （DW2021001-13）
重庆市技术创新与应用发展专项重点项目(CSTB2022TIAD-LUX0001) （CSTB2022TIAD-LUX0001）
山东省牛产业技术体系项目(SDAIT-09-11) （SDAIT-09-11）
