动物医学进展2025，Vol.46Issue(8)：51-57,7.
基于网络药理学和分子对接探究丹参治疗血淤证的分子机制
Exploring the Molecular Mechanism of Salvia miltiorrhiza in the Treatment of Blood Stasis Based on Network Pharmacology and Molecular Docking
摘要
Abstract
In this study,we investigated the molecular mechanism of Salvia miltiorrhiza in the treatment of blood stasis by network pharmacology and molecular docking.Firstly,we searched the targets of tanshi-none Ⅱ A(T Ⅱ A),cryptotanshinone(CT),salvianolic acid A(SAA),salvianolic acid B(SAB),tanshinoneⅠ(T Ⅰ)and dihydrotanshinone Ⅰ(DⅠ),the active ingredients of Salvia miltiorrhiza,through TCMSP and ETCM databases,and then screened the targets by using STRING database and Cystoscape software.Then,GO enrichment analysis,KEGG enrichment analysis and molecular docking were used to predict the biological processes,metabolic pathways and binding activities between the active ingredients of Salvia miltiorrhiza and their targets in the treatment of blood stasis syndrome.The GO functional enrichment a-nalysis of the retrieved targets of the active ingredients of Salvia miltiorrhiza showed that the biological processes associated with blood stasis were the cellular response to vascular endothelial growth stimulating factor(VEGF)and the vascular endothelial growth factor receptor(VEGFR)signaling pathway;the cellu-lar components associated with blood stasis were platelet α-granule region,ficolin-1-rich granule region,and blood microparticles;and the molecular functions associated with blood stasis were nitric oxide synthase(NO synthase),and KEGG pathway,and the regulatory activity function.KEGG pathway enrichment anal-ysis enriched three pathways,of which the pathway associated with blood stasis was the platelet activation pathway.The molecular docking results showed that T Ⅱ A,CT,SAA and SAB were tightly bound to the targets p38 and ERK,and SAA,SAB and DⅠ were tightly bound to the target SRC.This study demonstra-ted that the active ingredients of Salvia miltiorrhiza could inhibit platelet aggregation through the targets p38,ERK and SRC to treat blood stasis syndrome.关键词
网络药理学/分子对接/丹参/血淤证Key words
Network pharmacology/Molecular docking/Salvia miltiorrhiza/Blood stasis syndrome分类
农业科技引用本文复制引用
周迎晨,封彦飞,田诗旸,郭婷婷,边巴卓玛,刘迎秋..基于网络药理学和分子对接探究丹参治疗血淤证的分子机制[J].动物医学进展,2025,46(8):51-57,7.基金项目
陕西省农业关键核心技术攻关项目(2024NYGG005) （2024NYGG005）
