南方医科大学学报2025，Vol.45Issue(7)：1380-1388,9.DOI:10.12122/j.issn.1673-4254.2025.07.04
艾灸通过调控miR-223-3p/NLRP3焦亡通路修复薄型子宫内膜
Moxibustion promotes endometrial repair in rats with thin endometrium by inhibiting the NLRP3/pyroptosis axis via upregulating miR-223-3p
摘要
Abstract
Objective To explore the mechanism through which moxibustion promotes endometrial repair in rats with in thin endometrium(TE).Methods Female SD rats were randomized into control group,95%anhydrous ethanol-induced TE model group and moxibustion(at"Guan Yuan")group.High-throughput sequencing was used to identify the target genes of TE,and the targeting relationship between miR-223-3p and NLRP3 was verified using a dual luciferase assay.Histopathological of rat uterus was observed with HE staining,and expressions of miR-223-3p and NLRP3 were detected using RT-qPCR;serum levels of IL-1β and IL-18 of the rats were detected using ELISA,and protein expressions of NLRP3,ASC,caspase-1 and GSDMD in the uterus were detected with Western blotting.The pregnancies of the rats after treatment were counted.Results Enrichment analysis of the differential genes suggested up-regulated inflammatory response in TE,and dual luciferase assay verified targeted inhibition of NLRP3 expression by miR-223-3p.The rat models of TE had significantly decreased endometrial thickness and reduced endometrial glands and blood vessels with enhanced mRNA expression of NLRP3,increased serum levels of IL-1β and IL-18,up-regulated protein expressions of NLRP3,ASC,caspase-1 and GSDMD,lowered pregnancy rates on both the affected and unaffected sides and the overall number of pregnancies.Treatment of the rat models with mo-xibustion obviously increased the endometrial thickness and the density of glands and blood vessels,up-regulated miR-223-3p expression,lowered serum IL-1β and IL-18 levels and the protein expressions of NLRP3,ASC,caspase-1 and GSDMD,and significantly increased the number of pregnancies.Conclusion Moxibustion at"Guan Yuan"acupoint up-regulates the expression of miR-223-3p,which results in targeted inhibition of NLRP3 to suppress pyroptosis and promote endometrial repair in rat models of TE.关键词
薄型子宫内膜/中医药/艾灸/关元穴/细胞焦亡/miR-223-3p/NLRP3/pyroptosis信号轴Key words
thin endometrium/Chinese medicine/moxibustion/Guan Yuan acupoint/pyroptosis/miR-223-3p/NLRP3/pyroptosis signaling pathway引用本文复制引用
周海忆,何斯怡,韩瑞芳,关永格,董丽娟,宋阳..艾灸通过调控miR-223-3p/NLRP3焦亡通路修复薄型子宫内膜[J].南方医科大学学报,2025,45(7):1380-1388,9.基金项目
广东省自然科学基金(2024A1515012194) （2024A1515012194）
国家中医药传承发展示范试点项目-中山市中医院中医药科研立项(YN2024B008) （YN2024B008）
