南方医科大学学报2025，Vol.45Issue(7)：1434-1441,8.DOI:10.12122/j.issn.1673-4254.2025.07.09
针康结合促进C57/BL6J小鼠脑缺血后星形胶质细胞转分化为神经元
Electroacupuncture combined with rehabilitation training improves neurological function of mice with cerebral ischemia by promoting astrocyte transdifferentiation
摘要
Abstract
Objective To explore the effects of acupuncture combined with rehabilitation training for promoting transdifferentiation of astrocytes into neurons in mice after cerebral ischemia.Methods Male C57/BL6J mice were subjected to intracerebral microinjection of an adeno-associated virus carrying the GFAP promoter for NeuroD1 and Ngn2 overexpression in the astrocytes,followed 3 or 12 days later by electrocoagulation of the distal middle cerebral artery.After modeling,the mice were randomly divided into model group without interventions and intervention group treated with electroacupuncture at the acupoints Baihui(GV20),left Hegu(LI4),Neiguan(PC6),Zusanli(ST36),and Yanglingquan(GB34)24 h after surgery.The mice in the intervention group were housed individually in cages with running wheels,and their activity was recorded every 24 h.Neurological function scores of the mice were assessed on the 1st,14th,and 21st days after modeling.Transdifferentiation of astrocytes in the target brain regions was observed using double immunofluorescence staining.Results Compared with those in the model group,the mice receiving eletroacupuncture and rehabilitation training showed significant improvement of neurological deficits at 14 and 21 days after modeling.The GFAP promoter of the AAV2/5 vector specifically labeled the local astrocytes,and compared with that that in the model group,the number of AAV-positive cells colabeled with the neuronal marker DCX significantly increased after 14 days of electroacupuncture and rehabilitation intervention,and the number of AAV-positive cells colabeled with the neuronal marker NeuN significantly increased after 21 days of intervention.Conclusion In mice with cerebral ischemia,electroacupuncture and rehabilitation training can promote transdifferentiation of astrocytes into neurons in the ischemic brain region,and the efficiency of transdifferentiation is positively correlated with the improvement of motor function.关键词
星形胶质细胞/脑缺血/转分化/针康结合Key words
astrocytes/cerebral ischemia/transdifferentiation/electroacupuncture combined with rehabilitation training引用本文复制引用
唐东宁,康赟赟,何文杰,夏青..针康结合促进C57/BL6J小鼠脑缺血后星形胶质细胞转分化为神经元[J].南方医科大学学报,2025,45(7):1434-1441,8.基金项目
国家自然科学基金青年科学基金(82105021)Supported by Natural Science Foundation for the Youth(NSFY)of China(82105021). （82105021）
