南方医科大学学报2025，Vol.45Issue(7)：1442-1450,9.DOI:10.12122/j.issn.1673-4254.2025.07.10
贵州穿青人微单倍型遗传背景分析与法医学效能评估
Forensic performance and genetic background analyses of Guizhou Chuanqing population using a self-constructed microhaplotype panel
摘要
Abstract
Objective To investigate the ethnic origin of Chuanqing people,one of the largest unidentified ethnic groups in Guizhou,China,and analyze its genetic relationships with surrounding populations.Methods Based on a self-developed microhaplotype system,we conducted genotyping and analyzed the genetic distribution of microhaplotype loci and their forensic applicability in Chuanqing population in Guizhou Province.Using the microhaplotype data from different intercontinental populations and previously reported data from Han population living in Guizhou Province,we systematically investigated the genetic background of Chuanqing people through population genetic approaches,including genetic distance estimation,principal component analysis,and phylogenetic tree construction.Results Among the studied population,the number of haplotype per microhaplotype ranged from 6 to 25.The average expected heterozygosity(He),observed heterozygosity(Ho),power of discrimination(PD),and probability of exclusion(PE)were 0.8291,0.8301,0.9387,and 0.6593,respectively.The cumulative power of discrimination(CPD)and cumulative probability of exclusion(CPE)for these 33 loci were 1-2.62×10-41 and 1-7.64×10-17,respectively.Population genetic analyses revealed that the Chuanqing population had close genetic relationships with the East Asian populations,especially the local Guizhou Han population,Beijing Han population and the Han populations living in southern China.Conclusion The 33 microhaplotypes exhibit high levels of genetic diversity in the Guizhou Chuanqing population,highlighting their potentials for both forensic identification and parentage testing.The Han populations might have contributed a significant amount of genetic material to the Chuanqing population during the formation and development of the latter.关键词
穿青人/微单倍型/遗传结构/法医学效能Key words
Chuanqing population/microhaplotype/genetic structure/forensic performance引用本文复制引用
张红玲,杨美庆,季晶焱,靳小业,谷长云,王启燕,黄晓岚,冉乾冲,任峥,刘玉波,罗艳莎,潘帅吉..贵州穿青人微单倍型遗传背景分析与法医学效能评估[J].南方医科大学学报,2025,45(7):1442-1450,9.基金项目
国家自然科学基金(82160324) （82160324）
贵州省科技计划项目(ZK[2024],162,ZK[2022],355) （ZK[2024],162,ZK[2022],355）
贵州医科大学国家自然科学基金培育项目(21NSFCP02) （21NSFCP02）
贵州省大学生创新创业训练计划项(S202310660094) （S202310660094）
贵州省研究生科研基金项目(2024YJSKYJJ294)Supported by National Natural Science Foundation of China(82160324) （2024YJSKYJJ294）
