中国耳鼻咽喉头颈外科2025，Vol.32Issue(6)：366-370,5.DOI:10.16066/j.1672-7002.2025.06.006
嗜酸型慢性鼻-鼻窦炎伴鼻息肉小鼠模型免疫学特征表现
Immunological characteristics of a mouse model of eosinophilic chronic rhinosinusitis with nasal polyps
摘要
Abstract
OBJECTIVE Aimed to comprehensively evaluate various immunological changes in a mouse model of eosinophilic chronic rhinosinusitis with nasal polyps(ECRSwNP)induced by ovalbumin(OVA)combined with Aspergillus protease(AP),including nasal polyps-like lesions,subepithelial collagen deposition,inflammatory cell infiltration,epithelial barrier function,and olfactory neuron damage.METHODS C57BL/6 mice were challenged with OVA and AP for 12 weeks.Hematoxylin-eosin(HE),periodic acid-Schiff(PAS),and Masson staining were used to observe the changes of nasal polyps-like lesions,goblet cell hyperplasia,and subepithelial collagen deposition.Immunohistochemistry staining(IHC)was employed to detect the changes of various inflammatory cells,olfactory neurons,and the expression of epithelial tight junction proteins in the nasal and sinus mucosa.RESULTS Compared to the control group,the OVA and AP group showed significantly increased epithelial thickness,noticeable nasal polyps-like lesions,marked subepithelial collagen deposition,and goblet cell hyperplasia in the nasal and sinus mucosa.IHC results revealed a significant increase in eosinophils,along with higher numbers of neutrophils,mast cells,and CD4+T cells in the OVA and AP group.Additionally,the expression of tight junction proteins ZO-1 and E-cadherin in the nasal and sinus mucosa and the area of OMP+olfactory neurons in the olfactory epithelium were significantly lower in the OVA and AP group compared to the control.CONCLUSION Continuous exposure to OVA combined with AP successfully induces ECRSwNP.The establishment of this model provides a foundation for further research into the pathogenesis and the evaluation of new therapeutic strategies for ECRSwNP.关键词
鼻窦炎/鼻息肉/疾病模型,动物/免疫学特征Key words
Sinusitis/Nasal Polyps/Disease Models,Animal/immunological characteristics引用本文复制引用
李艳,蒋天娇,曲朕男,张罗,蓝凤..嗜酸型慢性鼻-鼻窦炎伴鼻息肉小鼠模型免疫学特征表现[J].中国耳鼻咽喉头颈外科,2025,32(6):366-370,5.基金项目
国家自然科学基金(82271140) （82271140）
北京市优秀人才青年拔尖(2018000021223ZK12) （2018000021223ZK12）
