东南园艺2025，Vol.13Issue(2)：117-127,11.DOI:10.20023/j.cnki.2095-5774.2025.02.001
基于HS-SPME-GC-MS联用技术的茉莉花挥发性代谢组学特征解析
HS-SPME-GC-MS analysis of volatile metabolomics profile in Fuzhou jasmine flower
摘要
Abstract
[Objective]This study aimed to systematically analyze the compositional characteristics of volatile compounds in Fuzhou jasmine flowers,identify key aroma-contributing substances,and explore their biosynthetic metabolic pathways,thereby providing a theoretical basis for understanding the molecular mechanisms of jasmine aroma formation.[Method]Headspace solid-phase microextraction(Headspace solid-phase microextraction,HS-SPME)coupled with gas chromatography-mass spectrometry(Gas chromatography-mass spectrometry,GC-MS)was employed to analyze the volatile components of Fuzhou double-petaled jasmine flowers.Semi-quantitative analysis,odor activity value(Odor activity value,OAV)calculation,and principal component analysis(Principal component analysis,PCA)were applied to evaluate key aroma compounds.The interaction of aroma substances was analyzed by correlation heat map,and the biosynthesis pathway of volatile components was further analyzed by metabolic pathway analysis.[Result]A total of 58 volatile organic compounds(VOCs)were identified,primarily including terpenes,esters,alcohols,and nitrogen-containing compounds,with linalool(14.65%),(Z)-3-hexenyl benzoate(12.12%),and α-farnesene(11.25%)being the most abundant.OAV and PCA analysis showed that α-farnesene and linalool had a key contribution to the characteristic aroma of jasmine flower,and terpenes were significantly positively correlated with esters,and the synergistic effect was beneficial to the release of jasmine aroma.Metabolic pathway analysis showed that the formation of jasmine aroma involved key metabolic networks such as secondary metabolite synthesis,sesquiterpene and triterpene compound formation.[Conclusion]The main aroma components in the volatiles of Fuzhou jasmine flower are linalool and α-farnesene.The synergistic effect of terpenes and esters is beneficial to enhance the aroma volatility.The synthesis of these volatile substances mainly depends on phenylalanine,tyrosine and tryptophan metabolic pathways.关键词
双瓣茉莉花/气相色谱-质谱联用/挥发性代谢组学/香气/主成分分析Key words
double-petaled jasmine flowers/HS-SPME-GC-MS/volatile metabolomics/aroma/principal component analysis分类
农业科技引用本文复制引用
张丽娜,任子文,徐玉英,黄彪,韦航,司瑞茹,傅晓萍,傅建炜..基于HS-SPME-GC-MS联用技术的茉莉花挥发性代谢组学特征解析[J].东南园艺,2025,13(2):117-127,11.基金项目
福建省人民政府与中国农业科学院农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021020) （XTCXGC2021020）
福州市科技创新平台项目(2022-P-017) （2022-P-017）
福建省属公益类科研院所基本科研专项(2021R1022002) （2021R1022002）
福建省农业科学院科技创新团队项目(CXTD2021011-1) （CXTD2021011-1）
