东南园艺2025，Vol.13Issue(2)：148-154,7.DOI:10.20023/j.cnki.2095-5774.2025.02.004
波罗蜜花粉生活力测定与花粉萌发培养基的优化
Determination of pollen viability and optimization of pollen germination medium in Artocarpus heterophyllus Lam
摘要
Abstract
[Objective]This study aimed to screen the optimal medium formulation for in vitro germination of Artrocarpus heterophyllus Lam.pollen and to evaluate the applicability of rapid staining method for detecting pollen viability.[Method]Pollen of'Siji'A.heterophyllus was used as experimental material to measure pollen germination rate and optimize pollen culture medium composition by single factor test and orthogonal test.[Result]The effects of sucrose,H3BO3,CaCl2 and MgSO4 on pollen germination rate were significant,with CaCl2 ranking first,followed by sucrose,H3BO3,and MgSO4.The optimum medium formula was 5%sucrose+150 mg/L H3BO3+100 mg/L CaCl2+150 mg/L MgSO4,and the germination rate was 72.39%,which was significantly higher than other treatments.The optimum germination temperature was 27℃.Both the MTT staining method(10 min staining,62.99%viability)and the TTC staining method(45 min staining,78.58%viability)could be used for rapid detection of pollen viability.The MTT staining method was more efficient and had better contrast ratio while the magenta acetate and I2-KI methods were not applicable.[Conclusion]The results provide a theoretical basis and practical support for pollen viability determination and artificial pollination technology in A.heterophyllus.cross breeding.关键词
波罗蜜/花粉/生活力/萌发/培养基/二苯基四氮唑溴盐染色法/氯化三苯四氮唑染色法Key words
Artocarpus heterophyllus/pollen/viability/germination/medium/MTT(2,5-diphenyl monotetrazolium bromide)staining/TTC(triphenyl tetrazolium chloride)staining分类
农业科技引用本文复制引用
何慧,吴伟豪,罗中君,谭俏茹,丰锋..波罗蜜花粉生活力测定与花粉萌发培养基的优化[J].东南园艺,2025,13(2):148-154,7.基金项目
广东省园艺一流专业建设点 （）
广东省植物生产试验教学示范中心 （）
云南省科技人才与平台计划项目"云南省丰锋专家工作站"(202305AF150146) （202305AF150146）
广东省波罗蜜种质资源圃(广东海大) （广东海大）
云南河口波罗蜜科技小院 （）
广东海洋大学大学生创新训练项目(S202410566027) （S202410566027）
