东南园艺2025，Vol.13Issue(2)：155-161,7.DOI:10.20023/j.cnki.2095-5774.2025.02.005
脂肪酶产生菌的分离鉴定及产酶条件优化
Isolation and identification of lipase producing bacteria and optimization of enzyme-producing conditions
摘要
Abstract
[Objective]The purpose of this experiment is to screen lipase producing bacteria and study their enzyme producing ability.[Method]The soil samples which had been contaminated by oil pollution for a long time,were enriched and cultured,and the strains were isolated and screened by Rhodamine B plate method.The shaking table culture with peanut oil as the only carbon source was used to explore the enzyme production parameters of the selected strains.The lipase production conditions of each strain were systematically optimized.[Result]Three strains with high enzyme production ability were obtained using Rhodamine B plate method and named as LN2,Bk3 and Bd4.Through by morphological and 16S rRNA molecular identification,LN2 was Pseudomonas aeruginosa,Bk3 was Burkholderia vietnamiensis and Bd4 was Acinetobacter soil.After 48 hours of cultivation,the optimal temperature for the three strains is 30～40℃,and the optimal culture pH is 7.0.Through comprehensive screening,the enzyme activity of LN2 was found to be 1.25 U/mL at 30℃and pH 7.0;the enzyme activity of Bk3 was 0.85 U/mL at 40℃and pH 7.0;and the enzyme activity of Bd4 was 3.13 U/mL at 30℃and pH 7.0.[Conclusion]The three lipase producing bacteria showed excellent potential in lipase production,especially Bd4,which provided candidate strain materials for the industrial application of lipase.关键词
脂肪酶/铜绿假单胞菌/越南伯克霍尔德氏菌/土壤不动杆菌/产酶温度Key words
lipase/Pseudomonas aeruginosa/Burkholderia vietnamiensis/Acinetobacter soil/optimal temperature分类
化学化工引用本文复制引用
李国风,周诗雯,程俊秋,李尊文,姜冉,洪瑛琪,许巧玲,叶炜..脂肪酶产生菌的分离鉴定及产酶条件优化[J].东南园艺,2025,13(2):155-161,7.基金项目
福建省科技计划项目(2023S0018、2023N0047) （2023S0018、2023N0047）
三明市科技计划项目(2022-N-24、2023-N-6、2023-N-19) （2022-N-24、2023-N-6、2023-N-19）
